Research Article
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Year 2023, Volume: 7 Issue: 3, 488 - 499, 30.09.2023

Flavien Shimira Ecem Kara Hatıra Taşkın

https://doi.org/10.31015/jaefs.2023.3.3

Abstract

Project Number

FDK-2020-13106

References

  • Alpsoy, H.C., and Şeniz, V. (2007). Researches on the in vitro Androgenesis and Obtaining Haploid Plants in Some Eggplant Genotypes. Acta Horticulturae, 729, 137–141. https://doi.org/10.17660/ActaHortic.2007.729.21
  • Başay, S., and Ellialtıoğlu, Ş.Ş. (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology, 37, 499–505. https://doi.org/10.3906/biy-1210-38
  • Bașay, S., Șeniz, V., and Ellialtıoğlu, Ș. (2011). Obtaining dihaploid lines by using anther culture in the different eggplant cultivars. Journal of Food, Agriculture & Environment, 9, 188-190.
  • Calabuig-Serna, A., Porcel, R., Corral-Martínez, P., and Seguí-Simarro, J. M. (2020). Anther Culture in Eggplant (Solanum melongena L.). In: Plant Embryogenesis. Methods in Molecular Biology, Volume 2122, Humana Press, New York, 283293.
  • Dumas De Vaulx, R., and Chambonnet, D. (1982). Culture in vitro d’anthères d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitments à +35°C associés à de faibles teneurs en substances de croissance. Agronomie, 2(10), 983–988.
  • Emrani Dehkehan, M., Moieni, A., and Movahedi, Z. (2017). Effects of zeatin riboside, mannitol and heat stress on eggplantn (Solanum melongena L.) anther culture. Iranian Journal of Genetics and Plant Breeding, 6(1), 16–26.
  • Kashyap, V., Kumar, S. V., Collonnier, C., Fusari, F., Haicour, R., Rotino, G. L., Sihachakr, D., and Rajama, M. V. (2003). Biotechnology of eggplant. Scientia Horticulturae, 97 (1), 1–25. https://doi.org/10.1016/S0304-4238(02)00140-1
  • Khatun, F., Meah, M. B., and Nasiruddin, K. M. (2006). Regeneration of eggplant through anther culture. Pakistan Journal of Biological Sciences, 9, 48–53.
  • Kumar, S., Singh, M., Prabhavathi, K., Mathews, A., Kumar, S., Singh, M., and Mathews, A. (2003). In vitro induction of haploid in eggplant (Solanum melongena L.). Capsicum and Eggplant Newsletter, 22, 147–150.
  • Mir, R., Calabuig-Serna, A., and Seguí-Simarro, J. M. (2021). Doubled haploids in eggplant. Biology, 10(7), 685. https://doi.org/10.3390/biology10070685
  • Rotino, G. L. (2016). Anther Culture in Eggplant (Solanum melongena L.). In: In vitro embryogenesis in higher plants, Methods in Molecular Biology, Volume 1359, Humana Press, New York, 453–466.
  • Salas, P., Prohens, J., and Seguí-Simarro, J.M. (2011). Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica, 182, 261–274. https://doi.org/10.1007/s10681-011-0490-2
  • Salas, P., Rivas-Sendra, A., Prohens, J., and Seguí-Simarro, J. M. (2012). Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica, 184(2), 235–250. https://doi.org/10.1007/s10681-011-0569-9
  • Seguí-Simarro, J.M. (2016). Androgenesis in Solanaceae (M. Germana, and M. Lambardi editors). In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY, 206–244.
  • Shimira, F., Boyaci, H. F., Çilesiz, Y., Nadeem, M. A., Baloch, F. S., and Taşkin, H. (2021). Exploring the genetic diversity and population structure of scarlet eggplant germplasm from Rwanda through iPBS-retrotransposon markers. Molecular Biology Reports, 48 (9), 6323–6333. https://doi.org/10.1007/s11033-021-06626-0
  • Shimira, F., Keleş, D., Taşkın, H., and Abak, K. (2019). The assessment of androgenic response of two nematode resistant pepper (Capsicum annuum L.) genotypes. Turkish Journal of Agriculture-Food Science and Technology, 7(12), 2103–2110. https://doi.org/10.24925/turjaf.v7i12.2103-2110.2828
  • Vural, G. E., and Ari, E. (2020). Triple synergistic effect of maltose, silver nitrate and activated charcoal on high embryo yield of eggplant (Solanum melongena L.) anther cultures. Scientia Horticulturae, 272, 109472. https://doi.org/10.1016/j.scienta.2020.109472
  • Vural, G. E., Ari, E., Zengin, S., and Ellialtioglu, S. S. (2019). Development of androgenesis studies on eggplant (Solanum melongena L.) in Turkey from past to present. In: Sustainable Crop Production, IntechOpen, London, 67–92

Pioneering anther culture-based embryogenesis in Solanum aethiopicum L.

Year 2023, Volume: 7 Issue: 3, 488 - 499, 30.09.2023

Flavien Shimira Ecem Kara Hatıra Taşkın

https://doi.org/10.31015/jaefs.2023.3.3

Abstract

This study aimed to establish a compelling approach for inducing embryogenesis through in vitro anther culture in Scarlet eggplant (Solanum aethiopicum L.), the most consumed and popular eggplant among indigenous vegetables on the African continent. While in vitro androgenesis has been favorably employed in brinjal (Solanum melongena L.) breeding, there has been no attempt to induce embryogenesis in a large germplasm of its relative, Solanum aethiopicum. In two distinct experiments, the largest germplasm collection of Solanum aethiopicum gr. Gilo was assessed for embryogenesis induction using C medium supplemented with different concentrations of hormones. In the first experiment, callus induction was successful with an overall rate of 36.6 calli/100 anthers, but embryo formation was unsuccessful. Statistical analysis revealed a dependency of the rate of callus induction on accessions. In the second experiment, only four selected accessions of Solanum aethiopicum gr. Gilo were used and compared to two Turkish eggplant genotypes of Solanum melongena in two distinct treatments. The results showed that in the first treatment (I), only the accession GKE12 had a satisfactory outcome with a rate of embryo formation of 0.82/100 anthers and 0.41/100 anthers corresponding to the rate of developed embryos. In the second treatment (II), only controls, which were Adana and Kemer cultivars of Solanum melongena formed embryos with a rate of 7.26/100 anthers and 1.15/100 anthers, respectively. The obtained embryo/seedling of Solanum aethiopicum gr. Gilo was found to be diploid. Overall, this study demonstrated that with the right combinations of hormones, it is possible to induce embryogenesis and produce a diploid of Solanum aethiopicum, the world’s second most popular cultivated eggplant after brinjal. These findings could potentially contribute to the breeding of eggplants for enhanced genetic variation and resistance.

Keywords

African eggplant Embryo formation In vitro androgenesis Microspore-derived embryos Plant breeding

Supporting Institution

Cukurova University—Scientific Research Projects Coordinating Office

Project Number

FDK-2020-13106

Thanks

Our sincere appreciation goes to Asst. Prof. Dr. Gökhan Baktemur, Dr. Şenay Karabıyık and Mr. Serhat Yıldız for guidance and technical assistance. We also extend our gratitude to Mahmut Yarar, Miyase Büyükküpeli, Fildaus Nyirahabimana, Ceren Mutludağ, Muhammed Halil Yutaz and Senem Uğur for their profound assistance during field and laboratory work. The authors are also grateful to Cukurova University—Scientific Research Projects Coordinating Office for financial support (Project No: FDK-2020-13106).

References

  • Alpsoy, H.C., and Şeniz, V. (2007). Researches on the in vitro Androgenesis and Obtaining Haploid Plants in Some Eggplant Genotypes. Acta Horticulturae, 729, 137–141. https://doi.org/10.17660/ActaHortic.2007.729.21
  • Başay, S., and Ellialtıoğlu, Ş.Ş. (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology, 37, 499–505. https://doi.org/10.3906/biy-1210-38
  • Bașay, S., Șeniz, V., and Ellialtıoğlu, Ș. (2011). Obtaining dihaploid lines by using anther culture in the different eggplant cultivars. Journal of Food, Agriculture & Environment, 9, 188-190.
  • Calabuig-Serna, A., Porcel, R., Corral-Martínez, P., and Seguí-Simarro, J. M. (2020). Anther Culture in Eggplant (Solanum melongena L.). In: Plant Embryogenesis. Methods in Molecular Biology, Volume 2122, Humana Press, New York, 283293.
  • Dumas De Vaulx, R., and Chambonnet, D. (1982). Culture in vitro d’anthères d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitments à +35°C associés à de faibles teneurs en substances de croissance. Agronomie, 2(10), 983–988.
  • Emrani Dehkehan, M., Moieni, A., and Movahedi, Z. (2017). Effects of zeatin riboside, mannitol and heat stress on eggplantn (Solanum melongena L.) anther culture. Iranian Journal of Genetics and Plant Breeding, 6(1), 16–26.
  • Kashyap, V., Kumar, S. V., Collonnier, C., Fusari, F., Haicour, R., Rotino, G. L., Sihachakr, D., and Rajama, M. V. (2003). Biotechnology of eggplant. Scientia Horticulturae, 97 (1), 1–25. https://doi.org/10.1016/S0304-4238(02)00140-1
  • Khatun, F., Meah, M. B., and Nasiruddin, K. M. (2006). Regeneration of eggplant through anther culture. Pakistan Journal of Biological Sciences, 9, 48–53.
  • Kumar, S., Singh, M., Prabhavathi, K., Mathews, A., Kumar, S., Singh, M., and Mathews, A. (2003). In vitro induction of haploid in eggplant (Solanum melongena L.). Capsicum and Eggplant Newsletter, 22, 147–150.
  • Mir, R., Calabuig-Serna, A., and Seguí-Simarro, J. M. (2021). Doubled haploids in eggplant. Biology, 10(7), 685. https://doi.org/10.3390/biology10070685
  • Rotino, G. L. (2016). Anther Culture in Eggplant (Solanum melongena L.). In: In vitro embryogenesis in higher plants, Methods in Molecular Biology, Volume 1359, Humana Press, New York, 453–466.
  • Salas, P., Prohens, J., and Seguí-Simarro, J.M. (2011). Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica, 182, 261–274. https://doi.org/10.1007/s10681-011-0490-2
  • Salas, P., Rivas-Sendra, A., Prohens, J., and Seguí-Simarro, J. M. (2012). Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica, 184(2), 235–250. https://doi.org/10.1007/s10681-011-0569-9
  • Seguí-Simarro, J.M. (2016). Androgenesis in Solanaceae (M. Germana, and M. Lambardi editors). In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY, 206–244.
  • Shimira, F., Boyaci, H. F., Çilesiz, Y., Nadeem, M. A., Baloch, F. S., and Taşkin, H. (2021). Exploring the genetic diversity and population structure of scarlet eggplant germplasm from Rwanda through iPBS-retrotransposon markers. Molecular Biology Reports, 48 (9), 6323–6333. https://doi.org/10.1007/s11033-021-06626-0
  • Shimira, F., Keleş, D., Taşkın, H., and Abak, K. (2019). The assessment of androgenic response of two nematode resistant pepper (Capsicum annuum L.) genotypes. Turkish Journal of Agriculture-Food Science and Technology, 7(12), 2103–2110. https://doi.org/10.24925/turjaf.v7i12.2103-2110.2828
  • Vural, G. E., and Ari, E. (2020). Triple synergistic effect of maltose, silver nitrate and activated charcoal on high embryo yield of eggplant (Solanum melongena L.) anther cultures. Scientia Horticulturae, 272, 109472. https://doi.org/10.1016/j.scienta.2020.109472
  • Vural, G. E., Ari, E., Zengin, S., and Ellialtioglu, S. S. (2019). Development of androgenesis studies on eggplant (Solanum melongena L.) in Turkey from past to present. In: Sustainable Crop Production, IntechOpen, London, 67–92
There are 18 citations in total.

Details

Primary Language English
Subjects Tissue Engineering
Journal Section Research Articles
Authors

Flavien Shimira 0000-0003-3382-4068

Ecem Kara 0000-0002-0118-2673

Hatıra Taşkın 0000-0002-1784-4731

Project Number FDK-2020-13106
Publication Date September 30, 2023
Submission Date May 30, 2023
Acceptance Date June 23, 2023
Published in Issue Year 2023 Volume: 7 Issue: 3

Cite

APA Shimira, F., Kara, E., & Taşkın, H. (2023). Pioneering anther culture-based embryogenesis in Solanum aethiopicum L. International Journal of Agriculture Environment and Food Sciences, 7(3), 488-499. https://doi.org/10.31015/jaefs.2023.3.3
AMA Shimira F, Kara E, Taşkın H. Pioneering anther culture-based embryogenesis in Solanum aethiopicum L. int. j. agric. environ. food sci. September 2023;7(3):488-499. doi:10.31015/jaefs.2023.3.3
Chicago Shimira, Flavien, Ecem Kara, and Hatıra Taşkın. “Pioneering Anther Culture-Based Embryogenesis in Solanum Aethiopicum L”. International Journal of Agriculture Environment and Food Sciences 7, no. 3 (September 2023): 488-99. https://doi.org/10.31015/jaefs.2023.3.3.
EndNote Shimira F, Kara E, Taşkın H (September 1, 2023) Pioneering anther culture-based embryogenesis in Solanum aethiopicum L. International Journal of Agriculture Environment and Food Sciences 7 3 488–499.
IEEE F. Shimira, E. Kara, and H. Taşkın, “Pioneering anther culture-based embryogenesis in Solanum aethiopicum L”., int. j. agric. environ. food sci., vol. 7, no. 3, pp. 488–499, 2023, doi: 10.31015/jaefs.2023.3.3.
ISNAD Shimira, Flavien et al. “Pioneering Anther Culture-Based Embryogenesis in Solanum Aethiopicum L”. International Journal of Agriculture Environment and Food Sciences 7/3 (September 2023), 488-499. https://doi.org/10.31015/jaefs.2023.3.3.
JAMA Shimira F, Kara E, Taşkın H. Pioneering anther culture-based embryogenesis in Solanum aethiopicum L. int. j. agric. environ. food sci. 2023;7:488–499.
MLA Shimira, Flavien et al. “Pioneering Anther Culture-Based Embryogenesis in Solanum Aethiopicum L”. International Journal of Agriculture Environment and Food Sciences, vol. 7, no. 3, 2023, pp. 488-99, doi:10.31015/jaefs.2023.3.3.
Vancouver Shimira F, Kara E, Taşkın H. Pioneering anther culture-based embryogenesis in Solanum aethiopicum L. int. j. agric. environ. food sci. 2023;7(3):488-99.
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