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In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa

Year 2012, Volume: 71 Issue: 2, 121 - 127, 25.12.2012

Abstract

Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging
to the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are
no studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated
aseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½)
medium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented
with 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration
and after three weeks, actively growing calli appeared and proliferated. Induction of adventitious
shoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three
weeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This
protocol provides a successful propagation technique through indirect in vitro organogenesis from leaf
segments of C. arifolia.
Keywords: C. arifolia, endemic species, micropropagation

References

  • Akan P. (2007) Türkiye için Endemik Centaurea cariensis Boiss. Alttürleri Üzerinde Morfolojik ve Palinolojik Araştırmalar. Karaelmas Üniversitesi Fen Fakültesi Orman Mühendisliği Yüksek Lisans Tezi.
  • Bona M. and Aras A. (2008). Numerical Taxonomic Study on Some Centaurea L. Species . IUFS J ournal of Biology, 67(1): 55-63.
  • Cuenca S., Amo-Marco J.B. and Para R. (1999) Micropropagation from inflorescense stems of the Spanish endemic plant Centaurea paui Loscos ex Wilk. (Compositae). Plant Cell Report, 18: 674–679.
  • Cuenca S. and Amo-Marco J.B. (2000). In vitro propagation of Centaurea spachii from inflorescence stems. Plant Growth Regul., 30: 99–103.
  • Çelik S., Uysal İ., Menemen Y. and Karabacak E. (2005) Morphology, Anatomy, Ecology, Polen and Achen Structure of Centaurea consanguinea DC. (Sect. Acrolophus) in Turkey. International Journal of Botany, 11: 85-89.
  • Davis P.H. (1988) Flora of Turkey and The East Aegean Islands. Vol. 10, Edinburgh University Press, Edinburgh.
  • Ekim T., Koyuncu M., Vural M., Duman H., Aytaç Z. and Adıgüzel N. (2000) Türkiye Bitkileri Kırmızı Kitabı. Türkiye Tabiatını Koruma Derneği Yayını, Ankara.
  • Ertuğrul K., Uysal T., Garcia-Jacas N., Susanna A. and Garnatje T. (2004) The systematic position of Centaurea ensiformis and Centaurea isaurica from Turkey and the evolution of some characters in Centaurea. Israel Journal of Plant Sciences, 52: 257-263.
  • Flamini G., Stoppelli G., Morelli I., Ertugrul K., Dural H., Tugay O. and Demirelma H. (2004) Secondary metabolites from Centaurea isaurica from Turkey and their chemotaxonomical significance. Biochemical Systematics and Ecology, 32 : 553–557.
  • Güner A., Özhatay N., Ekim T. and Başer K.H.C. (2000) Flora of Turkey and The East Aegean Islands, Volume 11 Edinburgh: Edinburgh University Press.
  • Hammatt N. and Evans P.K. (1985) The in vitro propagation of an endangered species: Centaurea junoniana Svent. (Compositae). Horticultural Science, 60: 93–97.
  • Hosoki T.J. and Kimura D.J. (1997) Micropropagation of Centaurea macrocephala Pushk. Ex. Willd. by shoot axis splitting. Horticultural Science, 32(6): 1124-1125.
  • Iriondo J. M. and Pérez C. (1996) Micropropagation and in vitro storage of Centaurium rigualii Esteve (Gentianaceae). Israel Journal of Plant Sciences, 44: 115–12.
  • IUCN (2001) Red List Categories: Version 3.1. Prepared by ICUN Species Survival Commission, ICUN, Gland Switzerland and Cambridge, UK.
  • Kakegawa K., Hattori E., Koike K. and Takeda K. (1991) Induction of anthocyanin synthesis and related enzyme activities in cell cultures of Centaurea cyanus by UV-light irradiation. Phytochemistry, 30(7): 2271-2273.
  • Kurt S. and Erdag B. (2009) In vitro germination and axillary shoot propagation of Centaurea zeybekii. Biologia., 64(1): 97—101.
  • Mallon R., Rodríguez-Oubiña J. and González M.L. (2010) In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell, Tissue and Organ Culture, 101: 31–39.
  • Murashige T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.
  • Okay Y. and Günöz A. (2009) Golbasi’na endemic Centaurea tchihatcheffii Fisch. et. Mey. The effect on germination of seeds of some applications. Journal of Agricultural Science, 15(2): 119-126.
  • Okay Y. and Demir K. (2010) Critically endangered endemic Centaurea tchihatcheffii Fisch. and Mey. and its propagation possibilities. African Journal of Agricultural Research, 5(25): 3536-
  • Özel Ç.A., Khawar K.M., Uluğ A., Kizilates E. and Arslan O. (2008) Centaurea depressa Bieb. ‘nın adventif sürgün rejenerasyonu. 23-27 Haziran 2008, 19. Ulusal Biyoloji Kongresi Karadeniz Teknik Üniversitesi Trabzon.
  • Özel Ç.A., Khawar K.M., Mirici S., Ozcan S. and Arslan O. (2006a) Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey). Naturwissenschaften 93:511–517.
  • Özel Ç.A., Khawar K.M., Mırıcı S., Arslan O. and Özcan S. (2006b) Induction of ex vitro adventitious roots and soft wood cuttings of Centaurea tchihatcheffii Fisch. et Mey. using Indole 3-Butyric and α-Naphthalene acetic acid. International Journal of Agriculture and Biology, 8 (1): 66-69.
  • Özel Ç.A. (2002) Centaurea tchihatcheffii’nin in vitro çoğaltımı. Gazi Üniv. Fen Bilimleri Enstitüsü Yüksek Lisans Tezi.
  • Özhatay N. and Kültür Ş. (2006) Check-list of additional taxa to the Supplement Flora of Turkey III. Turkish Journal of Botany, 30: 281-316.
  • Özhatay N., Kültür Ş. and Aslan S. (2009) Checklist of additional taxa to the Supplement Flora of Turkey IV. Turkish Journal of Botany, 33: 191-226.
  • Perica M.U. (2003) In Vitro Propagation of Centaurea rupestris L. Acta Biologica Cracoviensia, 45(2): 127–130.
  • Pevalek-Kozlina B. (1998) In vitro propagation of Centaurea ragusina L. a Croatian endemic species. Acta Biologica Cracoviensia, 40: 21–24.
  • Takashi J.H. and Daisuke A.K. (1997) Micropropagation of Centaurea macrocephala Pushk. Ex. Willd. by shoot axis splitting. Horticultural Science, 32(6): 1124-1125.
  • Tanimoto S. and Ishioka N. (1991) Effects of gelling agents on adventitious organ induction and callus growth in some plant species [Perilla frutescens, Centaurea cyanus, Lilium longiflorum, Callistephus chinensis and Torenia fournieri]. Bulletin of the Faculty of Agriculture Saga University, 70: 61-66.
  • Tıpırdamaz R., Özkum D., Özbek N. and Ellialtıoglu S. (2006) Centaurea tchihatcheffii Fisch. et Mey.’s researches on the production through tissue culture. In: Bosgelmez A (Ed.), Golbasi Mogan Lake, Andesite Bowl, Centaurea Tchihatcheffii, our Office Press-Ankara, Turkey. pp.569-579.
  • Wagenitz in Davis P. H. (1975) Flora of Turkey and The East Aegean Islands. Vol. 5, Edinburgh University Press, Edinburgh, ISBN: 0 521 08717 1.
  • Yaman B. (2005) Türkiye’nin Endemik Türlerinden Centaurea Pestalozzae Boiss. (Compositae)’in Morfolojik, Anatomik ve Palinolojik Özellikleri. Ormancılık Dergisi, 1(2): 1-12.

Endemik Centaurea arifolia Boiss. bitkisinin in Vitro üretimi

Year 2012, Volume: 71 Issue: 2, 121 - 127, 25.12.2012

Abstract

Centaurea L. (Asteraceae) Türkiye florasının en geniş cinslerinden biridir. Centaurea cinsine ait olan Centaurea arifolia IUCN kategorisine göre DD (veri yetersiz) olarak değerlendirilmiştir. Bu tür ile ilgili herhangi bir in vitro propagasyon çalışmasına rastlanmamıştır. Bu çalışmada, tohumlar büyüme düzenleyicisi içermeyen 1\2 Murashige ve Skoog (MS ½) ortamında petri kaplarında aseptik koşullarda çimlendirilmiştir. Altı haftalık fidelerden alınan yaprak eksplantları dolaylı rejenerasyon için 1 mg l−1 BAP + 0.1 mg l−1 NAA ve 2 mg l−1 BAP + 0.2 mg l−1 NAA hormon kombinasyonlarını içeren MS ortamına transfer edilmişlerdir. Üç hafta sonra aktif olarak büyüyen kalluslar elde edilmiştir. Kalluslardan üç hafta sonra adventif gövde rejenerasyonunun teşviki yaklaşık olarak % 150 (MS1) ve % 120 (MS2) oranında başarı elde edilmiştir. Rejenere olmuş adventif gövdeler 1 mg l−1 IBA içeren MS ortamında köklendirilmiştir. Bu yöntem, C. arifolia’nın yaprak segmentlerinden in vitro dolaylı organogenesis aracılığı ile oluşturulmuş başarılı bir üretim tekniği sağlamaktadır.

References

  • Akan P. (2007) Türkiye için Endemik Centaurea cariensis Boiss. Alttürleri Üzerinde Morfolojik ve Palinolojik Araştırmalar. Karaelmas Üniversitesi Fen Fakültesi Orman Mühendisliği Yüksek Lisans Tezi.
  • Bona M. and Aras A. (2008). Numerical Taxonomic Study on Some Centaurea L. Species . IUFS J ournal of Biology, 67(1): 55-63.
  • Cuenca S., Amo-Marco J.B. and Para R. (1999) Micropropagation from inflorescense stems of the Spanish endemic plant Centaurea paui Loscos ex Wilk. (Compositae). Plant Cell Report, 18: 674–679.
  • Cuenca S. and Amo-Marco J.B. (2000). In vitro propagation of Centaurea spachii from inflorescence stems. Plant Growth Regul., 30: 99–103.
  • Çelik S., Uysal İ., Menemen Y. and Karabacak E. (2005) Morphology, Anatomy, Ecology, Polen and Achen Structure of Centaurea consanguinea DC. (Sect. Acrolophus) in Turkey. International Journal of Botany, 11: 85-89.
  • Davis P.H. (1988) Flora of Turkey and The East Aegean Islands. Vol. 10, Edinburgh University Press, Edinburgh.
  • Ekim T., Koyuncu M., Vural M., Duman H., Aytaç Z. and Adıgüzel N. (2000) Türkiye Bitkileri Kırmızı Kitabı. Türkiye Tabiatını Koruma Derneği Yayını, Ankara.
  • Ertuğrul K., Uysal T., Garcia-Jacas N., Susanna A. and Garnatje T. (2004) The systematic position of Centaurea ensiformis and Centaurea isaurica from Turkey and the evolution of some characters in Centaurea. Israel Journal of Plant Sciences, 52: 257-263.
  • Flamini G., Stoppelli G., Morelli I., Ertugrul K., Dural H., Tugay O. and Demirelma H. (2004) Secondary metabolites from Centaurea isaurica from Turkey and their chemotaxonomical significance. Biochemical Systematics and Ecology, 32 : 553–557.
  • Güner A., Özhatay N., Ekim T. and Başer K.H.C. (2000) Flora of Turkey and The East Aegean Islands, Volume 11 Edinburgh: Edinburgh University Press.
  • Hammatt N. and Evans P.K. (1985) The in vitro propagation of an endangered species: Centaurea junoniana Svent. (Compositae). Horticultural Science, 60: 93–97.
  • Hosoki T.J. and Kimura D.J. (1997) Micropropagation of Centaurea macrocephala Pushk. Ex. Willd. by shoot axis splitting. Horticultural Science, 32(6): 1124-1125.
  • Iriondo J. M. and Pérez C. (1996) Micropropagation and in vitro storage of Centaurium rigualii Esteve (Gentianaceae). Israel Journal of Plant Sciences, 44: 115–12.
  • IUCN (2001) Red List Categories: Version 3.1. Prepared by ICUN Species Survival Commission, ICUN, Gland Switzerland and Cambridge, UK.
  • Kakegawa K., Hattori E., Koike K. and Takeda K. (1991) Induction of anthocyanin synthesis and related enzyme activities in cell cultures of Centaurea cyanus by UV-light irradiation. Phytochemistry, 30(7): 2271-2273.
  • Kurt S. and Erdag B. (2009) In vitro germination and axillary shoot propagation of Centaurea zeybekii. Biologia., 64(1): 97—101.
  • Mallon R., Rodríguez-Oubiña J. and González M.L. (2010) In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell, Tissue and Organ Culture, 101: 31–39.
  • Murashige T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.
  • Okay Y. and Günöz A. (2009) Golbasi’na endemic Centaurea tchihatcheffii Fisch. et. Mey. The effect on germination of seeds of some applications. Journal of Agricultural Science, 15(2): 119-126.
  • Okay Y. and Demir K. (2010) Critically endangered endemic Centaurea tchihatcheffii Fisch. and Mey. and its propagation possibilities. African Journal of Agricultural Research, 5(25): 3536-
  • Özel Ç.A., Khawar K.M., Uluğ A., Kizilates E. and Arslan O. (2008) Centaurea depressa Bieb. ‘nın adventif sürgün rejenerasyonu. 23-27 Haziran 2008, 19. Ulusal Biyoloji Kongresi Karadeniz Teknik Üniversitesi Trabzon.
  • Özel Ç.A., Khawar K.M., Mirici S., Ozcan S. and Arslan O. (2006a) Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey). Naturwissenschaften 93:511–517.
  • Özel Ç.A., Khawar K.M., Mırıcı S., Arslan O. and Özcan S. (2006b) Induction of ex vitro adventitious roots and soft wood cuttings of Centaurea tchihatcheffii Fisch. et Mey. using Indole 3-Butyric and α-Naphthalene acetic acid. International Journal of Agriculture and Biology, 8 (1): 66-69.
  • Özel Ç.A. (2002) Centaurea tchihatcheffii’nin in vitro çoğaltımı. Gazi Üniv. Fen Bilimleri Enstitüsü Yüksek Lisans Tezi.
  • Özhatay N. and Kültür Ş. (2006) Check-list of additional taxa to the Supplement Flora of Turkey III. Turkish Journal of Botany, 30: 281-316.
  • Özhatay N., Kültür Ş. and Aslan S. (2009) Checklist of additional taxa to the Supplement Flora of Turkey IV. Turkish Journal of Botany, 33: 191-226.
  • Perica M.U. (2003) In Vitro Propagation of Centaurea rupestris L. Acta Biologica Cracoviensia, 45(2): 127–130.
  • Pevalek-Kozlina B. (1998) In vitro propagation of Centaurea ragusina L. a Croatian endemic species. Acta Biologica Cracoviensia, 40: 21–24.
  • Takashi J.H. and Daisuke A.K. (1997) Micropropagation of Centaurea macrocephala Pushk. Ex. Willd. by shoot axis splitting. Horticultural Science, 32(6): 1124-1125.
  • Tanimoto S. and Ishioka N. (1991) Effects of gelling agents on adventitious organ induction and callus growth in some plant species [Perilla frutescens, Centaurea cyanus, Lilium longiflorum, Callistephus chinensis and Torenia fournieri]. Bulletin of the Faculty of Agriculture Saga University, 70: 61-66.
  • Tıpırdamaz R., Özkum D., Özbek N. and Ellialtıoglu S. (2006) Centaurea tchihatcheffii Fisch. et Mey.’s researches on the production through tissue culture. In: Bosgelmez A (Ed.), Golbasi Mogan Lake, Andesite Bowl, Centaurea Tchihatcheffii, our Office Press-Ankara, Turkey. pp.569-579.
  • Wagenitz in Davis P. H. (1975) Flora of Turkey and The East Aegean Islands. Vol. 5, Edinburgh University Press, Edinburgh, ISBN: 0 521 08717 1.
  • Yaman B. (2005) Türkiye’nin Endemik Türlerinden Centaurea Pestalozzae Boiss. (Compositae)’in Morfolojik, Anatomik ve Palinolojik Özellikleri. Ormancılık Dergisi, 1(2): 1-12.
There are 33 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Elif Yüzbaşıoğlu

Eda Dalyan This is me

Mehmet Bona This is me

Gül Öz This is me

Publication Date December 25, 2012
Submission Date December 25, 2012
Published in Issue Year 2012 Volume: 71 Issue: 2

Cite

AMA Yüzbaşıoğlu E, Dalyan E, Bona M, Öz G. In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa. Eur J Biol. November 2012;71(2):121-127.