Research Article
Year 2022,
Volume: 81 Issue: 2, 184 - 189, 29.12.2022
Abstract
References
- 1. Karl TR, Kukla G, Razuvayev VN, Changery MJ, Quayle RG, Heim RR, Easterlinf DR, Fu CB. Global warming: evidence for asymmetric diurnal temperature change. Geophys Res Lett 1991; 18: 2253-6. google scholar
- 2. Pisani T, Paoli L, Gaggi C, Pirintsos SA, Loppi S. Effects of high temperature on epiphytic lichens: Issues for consideration in a changing climate scenario. Plant Biosyst 2007; 141: 164- 9. google scholar
- 3. Dyakov MY, Insarova ID, Kharabadze DE, Ptushenko VV, Shtaer OV. Influence of extreme ambient temperatures and anaerobic conditions on Peltigera aphthosa (L.) Wild. viability. Life Sci Space Res 2015; 7: 66-72. google scholar
- 4. Sanita di Toppi BL, Pawlik-Skowronska E, Vurro Z, Vattuone R, Ka-linowska FM. First and second line mechanisms of cadmium detoxification in the lichen photobiont Trebouxia impressa (Chlorophyta). Environ Pollut 2008; 151: 280-6. google scholar
- 5. Xu S, Li J, Zhang X, Wei H, Cui L. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplast in two-season turfgrass species under heat stress. Environ Exp Bot 2006; 56: 274-85. google scholar
- 6. Sen G, Kutlu E, Ozakca D. The effect of aluminium and exogenous spermidine on chlorophyll degradation, glutathione reductase activity and the photosystem II D1 protein gene (psbA) transcript level in lichen Xanthoria parietina. Phytochemistry 2014; 98: 54-9. google scholar
- 7. Youn YS, Park JK, Jang HD, Rhee YW. Sequential hydration with anaerobic and heat treatment increases GABA (Y-aminobutyric acid) contnt in wheat. Food Chem 2011; 129: 1631-5. google scholar
- 8. Nayyar H, Kaur, R, Kaur S, Singh R. Y-aminobutyric acid (GABA) imparts partial protection from heat stress injury to rice seedlings by improving leaf turgor and upregulating osmoprotectants and antioxidants. J Plant Growth Regul 2014; 33: 408-19. google scholar
- 9. Li Z, Yu J, Peng Y, Huang B. Metabolic pathways regulated by Y-am-inobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera). Sci Rep 2016; 6: 1-6. google scholar
- 10. Kinnersley AM, Turano FJ. Gamma Aminobutyric Acid (GABA) and Plant Responses to Stress. Crit Rev Plant Sci 2000; 19: 479-509. google scholar
- 11. Sharma SS, Dietz KJ. The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 2006; 57: 28711-26. google scholar
- 12. Chand N, Muhammad S, Khan RU, Alhidary IA, Rehman ZU. Ameliorative effect of synthetic Y-aminobutyric acid (GABA) on performance traits, antioxidant status and immune response in broiler exposed to cyclic heat stress. Environ Sci Pollut Res 2016; 23: 23930-5. google scholar
- 13. Fait A, Fromm H, Walter D, Galili G, Fernie AR. Highway or byway: the metabolic role of the GABA shunt in plants. Trends Plant Sci 2008; 13: 14-9. google scholar
- 14. Yolcu S, Ozdemir F, Bor M. Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phyloge-netically divergent plants. Plant Syst. Evol 2013;299: 403-12. google scholar
- 15. Bouché N, Fromm H. GABA in plants : just a metabolite ? Trends Plant Sci 2004;9:110-15. google scholar
- 16. Bouché N, Fait A, Bouchez D, Moller SG, Fromm H. Mitochondrial succinic-semialdehyde dehydrogenase of the gamma-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. Proc Natl Acad Sci U.S.A 2003; 100: 6843-8. google scholar
- 17. Bartyzel I, Pelczar K, Paszkowski A. Functioning of the gammaaminobutyrate pathway in wheat seedlings affected by osmotis stress. Biol Plant 2003; 47: 221-5. google scholar
- 18. Bor M, Seckin B, Ozgur R, Yilmaz O, Ozdemir F, Turkan I. Comparative effects of drought, salt, heavy metal and heat stresses on gam-ma-aminobutryric acid levels of sesame. Acta Physiol Plant 2009; 31: 655-9. google scholar
- 19. Al-Quraan NA, Locy RD, Singh NK. Implications of paraquat and hydrogen peroxide-induced oxidative stress treatments on the GABA shunt pathway in Arabidopsis thaliana calmodulin mutants. Plant Biotechnol Rep 2011; 5: 225-34. google scholar
- 20. Cao J, Barbosa JM, Singh N, Locy RD. GABA shunt mediates thermotolerance in Saccharomyces ceresiae by reducing reactive oxygen production. Yeast 2013; 30: 129-44. google scholar
- 21. Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 1994; 144: 30713. google scholar
- 22. Akihiro T, Koike S, Tani R, Tominaga T, Watanabe S, Iijima Y, et al. Biochemical mechanism on GABA accumulation during fruit development in tomato. Plant Cell Physiol 2008; 49: 1378-89. google scholar
- 23. Heath RL, Packer L. Photoperoxidation in isolated chloroplast: 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 1968; 125: 189-98. google scholar
- 24. Bergmeyer, N. Methoden der enzymatischen analyse. Akademie Verlag, Berlin 1970; 1: 636-64. google scholar
- 25. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 1981; 22: 867-80. google scholar
- 26. Birecka H, Briber KA, Catalfamo JL. Comparative studies on tobacco pith and sweet potato root isoperoxidases in relation to injury, indoleacetic acid, and ethylene effects. Plant Physiol 1973; 52: 439. google scholar
- 27. Bradford MM. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein dye binding. Anal Biochem 1976; 72: 248-54. google scholar
- 28. Karim MA, Fracheboud Y, Stamp P. Photosynthetic activity of developing leaves of Zea mays is less affected by heat stress than that of development leaves. Physiol Plant 1999; 105: 685-93. google scholar
- 29. Chettri MK, Cook CM, Vardaka E, Sawidis T, Lanaras T. The effect of Cu, Zn, and Pb on the chlorophyll content of lichens Cladonia convolute and Cladonia rangiformis. Environ Exp Bot 1998; 39: 1-10. google scholar
- 30. Backor M, Zetikova J. Effects of copper, cobalt and mercury on the chlorophyll content of lichens Centraria islandica and Flavocetraria cucullata. J Hattori Bot Lab 2003; 93: 175- 87. google scholar
- 31. Unal D, Tuney I, Sukatar A. The role of external polyamines on photosynthetic responses, lipid peroxidation, protein and chlorophyll a content under the UV-A (352 nm) stress in Physcia semipinnata. J Photochem Photobiol B, Biol 2008; 90: 64-8. google scholar
- 32. Cekic FO, Goren-Saglam N, Torun H, Yiğit E, Unal D. Gamma-Amino Butyric acid metabolism under high temperature stress in two lichen species. Appl Ecol Environ Res 2018; 16: 5529-38. google scholar
- 33. Cao S, Cai Y, Yang Z, Zheng Y. MeJA induces chilling tolerance in lo-quat fruit by regulating proline and Y-aminobutyric acid contents. Food Chem 2012; 133: 1466-70. google scholar
- 34. Andersson JO, Roger AJ. Evolution of GDH genes: evidence for lateral gene transfer within and between prokaryotes and eukaryotes. BMC Evol Biol 2003; 3: 14. google scholar
- 35. Forde BG, Lea PJ. Glutamate in plants: metabolism, regulation and signalling. J Exp Bot 2007; 58: 2339-58. google scholar
- 36. Sadowsky A, Mettler-Altmann T, Ott S. Metabolic response to desiccation stress in strains of green algal photobionts (Trebouxia) from two Antarctic lichens of southern habitats. Phycologia 2016; 55: 703-14. google scholar
- 37. Cowan DA, Wilson AT, Green TGA. Lichen metabolism: 2. Aspects of light and dark physiology. New Phytol 1979; 83: 761-9. google scholar
- 38. Bouché N, Fait A, Zik M, Fromm H. The root-specific glutamate decarboxylase (GAD1) is essential for sustaining GABA levels in Ara-bidopsis. Plant Mol Biol 2004; 55: 315-25. google scholar
- 39. Xu Y, Xiao H, Guan H, Wang Y, Long C, Zhao J. Variations in free amino acid concentrations in mosses and different parts of Cinnamo-mum camphora along an urban-to-rural gradient. Ecol Indic 2018; 93: 813-21. google scholar
- 40. Baek KH, Skinner DZ. Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Sci 2003; 165: 1221-7. google scholar
The Role of Gamma-Amino Butyric Acid in Short-Term High Temperature Acclimation in Lichen Pseudevernia furfuracea
Abstract
Objective: Global warming causes many different stresses for plants. High-temperature stress is one of the important problems caused by global warming. Plants develop various tolerance mechanisms to protect themselves against these stresses. γ-Aminobutyric acid (GABA) metabolism has a critical role in various biological processes in plants. GABA plays a critical role in the acclimation to different stress conditions in plants. Lichens can grow in environments exposed to severe abiotic stresses such as drought and extreme heat. The major aim of this study was to identify whether GABA accumulation could improve short-term, high-temperature tolerance in lichen Pseudevernia furfuracea. Materials and Methods: For this aim, P. furfuracea samples were kept in petri dishes in an incubator at 45 ± 2°C for 24 and 48 h. We analyzed the chlorophyll a/b ratio, GABA content, glutamate decarboxylase (GAD) and glutamate dehydrogenase (GDH) activities, which are important enzymes involved in the GABA shunt, and also peroxidase (POD) and catalase (CAT) activities of the antioxidant metabolism. Results: Our study indicated that the chlorophyll a/b ratio was not changed significantly under 45°C within 48 h. POD and CAT activities were significantly decreased in lichen thalli under 45°C, however; GABA accumulation was approximately enhanced by 1.5-fold depending on the time exposure. GAD and GDH activities were significantly increased under high temperature conditions. Conclusions: The acclimilation of P. furfuracea to high temperatures may be related to the increase in GAD and GDH activities. Our findings provided evidence that the GABA shunt could help lichen P. furfuracea to acclimate to high temperatures.
References
- 1. Karl TR, Kukla G, Razuvayev VN, Changery MJ, Quayle RG, Heim RR, Easterlinf DR, Fu CB. Global warming: evidence for asymmetric diurnal temperature change. Geophys Res Lett 1991; 18: 2253-6. google scholar
- 2. Pisani T, Paoli L, Gaggi C, Pirintsos SA, Loppi S. Effects of high temperature on epiphytic lichens: Issues for consideration in a changing climate scenario. Plant Biosyst 2007; 141: 164- 9. google scholar
- 3. Dyakov MY, Insarova ID, Kharabadze DE, Ptushenko VV, Shtaer OV. Influence of extreme ambient temperatures and anaerobic conditions on Peltigera aphthosa (L.) Wild. viability. Life Sci Space Res 2015; 7: 66-72. google scholar
- 4. Sanita di Toppi BL, Pawlik-Skowronska E, Vurro Z, Vattuone R, Ka-linowska FM. First and second line mechanisms of cadmium detoxification in the lichen photobiont Trebouxia impressa (Chlorophyta). Environ Pollut 2008; 151: 280-6. google scholar
- 5. Xu S, Li J, Zhang X, Wei H, Cui L. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplast in two-season turfgrass species under heat stress. Environ Exp Bot 2006; 56: 274-85. google scholar
- 6. Sen G, Kutlu E, Ozakca D. The effect of aluminium and exogenous spermidine on chlorophyll degradation, glutathione reductase activity and the photosystem II D1 protein gene (psbA) transcript level in lichen Xanthoria parietina. Phytochemistry 2014; 98: 54-9. google scholar
- 7. Youn YS, Park JK, Jang HD, Rhee YW. Sequential hydration with anaerobic and heat treatment increases GABA (Y-aminobutyric acid) contnt in wheat. Food Chem 2011; 129: 1631-5. google scholar
- 8. Nayyar H, Kaur, R, Kaur S, Singh R. Y-aminobutyric acid (GABA) imparts partial protection from heat stress injury to rice seedlings by improving leaf turgor and upregulating osmoprotectants and antioxidants. J Plant Growth Regul 2014; 33: 408-19. google scholar
- 9. Li Z, Yu J, Peng Y, Huang B. Metabolic pathways regulated by Y-am-inobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera). Sci Rep 2016; 6: 1-6. google scholar
- 10. Kinnersley AM, Turano FJ. Gamma Aminobutyric Acid (GABA) and Plant Responses to Stress. Crit Rev Plant Sci 2000; 19: 479-509. google scholar
- 11. Sharma SS, Dietz KJ. The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 2006; 57: 28711-26. google scholar
- 12. Chand N, Muhammad S, Khan RU, Alhidary IA, Rehman ZU. Ameliorative effect of synthetic Y-aminobutyric acid (GABA) on performance traits, antioxidant status and immune response in broiler exposed to cyclic heat stress. Environ Sci Pollut Res 2016; 23: 23930-5. google scholar
- 13. Fait A, Fromm H, Walter D, Galili G, Fernie AR. Highway or byway: the metabolic role of the GABA shunt in plants. Trends Plant Sci 2008; 13: 14-9. google scholar
- 14. Yolcu S, Ozdemir F, Bor M. Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phyloge-netically divergent plants. Plant Syst. Evol 2013;299: 403-12. google scholar
- 15. Bouché N, Fromm H. GABA in plants : just a metabolite ? Trends Plant Sci 2004;9:110-15. google scholar
- 16. Bouché N, Fait A, Bouchez D, Moller SG, Fromm H. Mitochondrial succinic-semialdehyde dehydrogenase of the gamma-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. Proc Natl Acad Sci U.S.A 2003; 100: 6843-8. google scholar
- 17. Bartyzel I, Pelczar K, Paszkowski A. Functioning of the gammaaminobutyrate pathway in wheat seedlings affected by osmotis stress. Biol Plant 2003; 47: 221-5. google scholar
- 18. Bor M, Seckin B, Ozgur R, Yilmaz O, Ozdemir F, Turkan I. Comparative effects of drought, salt, heavy metal and heat stresses on gam-ma-aminobutryric acid levels of sesame. Acta Physiol Plant 2009; 31: 655-9. google scholar
- 19. Al-Quraan NA, Locy RD, Singh NK. Implications of paraquat and hydrogen peroxide-induced oxidative stress treatments on the GABA shunt pathway in Arabidopsis thaliana calmodulin mutants. Plant Biotechnol Rep 2011; 5: 225-34. google scholar
- 20. Cao J, Barbosa JM, Singh N, Locy RD. GABA shunt mediates thermotolerance in Saccharomyces ceresiae by reducing reactive oxygen production. Yeast 2013; 30: 129-44. google scholar
- 21. Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 1994; 144: 30713. google scholar
- 22. Akihiro T, Koike S, Tani R, Tominaga T, Watanabe S, Iijima Y, et al. Biochemical mechanism on GABA accumulation during fruit development in tomato. Plant Cell Physiol 2008; 49: 1378-89. google scholar
- 23. Heath RL, Packer L. Photoperoxidation in isolated chloroplast: 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 1968; 125: 189-98. google scholar
- 24. Bergmeyer, N. Methoden der enzymatischen analyse. Akademie Verlag, Berlin 1970; 1: 636-64. google scholar
- 25. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 1981; 22: 867-80. google scholar
- 26. Birecka H, Briber KA, Catalfamo JL. Comparative studies on tobacco pith and sweet potato root isoperoxidases in relation to injury, indoleacetic acid, and ethylene effects. Plant Physiol 1973; 52: 439. google scholar
- 27. Bradford MM. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein dye binding. Anal Biochem 1976; 72: 248-54. google scholar
- 28. Karim MA, Fracheboud Y, Stamp P. Photosynthetic activity of developing leaves of Zea mays is less affected by heat stress than that of development leaves. Physiol Plant 1999; 105: 685-93. google scholar
- 29. Chettri MK, Cook CM, Vardaka E, Sawidis T, Lanaras T. The effect of Cu, Zn, and Pb on the chlorophyll content of lichens Cladonia convolute and Cladonia rangiformis. Environ Exp Bot 1998; 39: 1-10. google scholar
- 30. Backor M, Zetikova J. Effects of copper, cobalt and mercury on the chlorophyll content of lichens Centraria islandica and Flavocetraria cucullata. J Hattori Bot Lab 2003; 93: 175- 87. google scholar
- 31. Unal D, Tuney I, Sukatar A. The role of external polyamines on photosynthetic responses, lipid peroxidation, protein and chlorophyll a content under the UV-A (352 nm) stress in Physcia semipinnata. J Photochem Photobiol B, Biol 2008; 90: 64-8. google scholar
- 32. Cekic FO, Goren-Saglam N, Torun H, Yiğit E, Unal D. Gamma-Amino Butyric acid metabolism under high temperature stress in two lichen species. Appl Ecol Environ Res 2018; 16: 5529-38. google scholar
- 33. Cao S, Cai Y, Yang Z, Zheng Y. MeJA induces chilling tolerance in lo-quat fruit by regulating proline and Y-aminobutyric acid contents. Food Chem 2012; 133: 1466-70. google scholar
- 34. Andersson JO, Roger AJ. Evolution of GDH genes: evidence for lateral gene transfer within and between prokaryotes and eukaryotes. BMC Evol Biol 2003; 3: 14. google scholar
- 35. Forde BG, Lea PJ. Glutamate in plants: metabolism, regulation and signalling. J Exp Bot 2007; 58: 2339-58. google scholar
- 36. Sadowsky A, Mettler-Altmann T, Ott S. Metabolic response to desiccation stress in strains of green algal photobionts (Trebouxia) from two Antarctic lichens of southern habitats. Phycologia 2016; 55: 703-14. google scholar
- 37. Cowan DA, Wilson AT, Green TGA. Lichen metabolism: 2. Aspects of light and dark physiology. New Phytol 1979; 83: 761-9. google scholar
- 38. Bouché N, Fait A, Zik M, Fromm H. The root-specific glutamate decarboxylase (GAD1) is essential for sustaining GABA levels in Ara-bidopsis. Plant Mol Biol 2004; 55: 315-25. google scholar
- 39. Xu Y, Xiao H, Guan H, Wang Y, Long C, Zhao J. Variations in free amino acid concentrations in mosses and different parts of Cinnamo-mum camphora along an urban-to-rural gradient. Ecol Indic 2018; 93: 813-21. google scholar
- 40. Baek KH, Skinner DZ. Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Sci 2003; 165: 1221-7. google scholar
There are 40 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 29, 2022 |
| Submission Date | August 4, 2022 |
| Published in Issue | Year 2022 Volume: 81 Issue: 2 |
