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Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress

Year 2023, Volume: 10 Issue: 4, 570 - 582, 01.12.2023

Muhammed Mustafa Polat Ergun Kaya İbrahim Kıvrak

https://doi.org/10.21448/ijsm.1309982

Abstract

In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.

Keywords

Boric acid Salt stress Sodium metaborate Liquidambar orientalis Phenolic compounds

References

  • Acar, M.I. (1989). Determination of composition of Liquidambar orientalis Mill. balm essential oil by analyzing it with GC-MS-DS system. Forestry Research Institute Publications, 33, 5-21.
  • Alan, M., & Kaya, Z. (2003). Oriental sweet gum Liquidambar orientalis. EUFORGEN technical guidelines. www.biodiversityinternational.org/publications
  • Alan, M., Velioglu, E., Ezen, T., Sıklar, S., & Ozturk, H. (2018). Diversity of some quantitative characters of oriental sweet gum (Liquidambar orientalis Mill.) for five-year-old seedlings. Turkish Journal of Forestry Research, 5(1), 74-81.
  • Baranek, M., Krizan, B., Ondrusikova, E., & Pidra, M. (2010). DNA-methylation changes in grapevine somaclones follow in vitro culture and thermotherapy. Plant Cell, Tissue and Organ Culture, 101(1), 11-22. https://doi.org/10.1007/s11240-009-9656-1
  • Barret, P., Brinkman, M., & Beckert, M. (2006). A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. Genome, 49(11), 1399-1407.
  • Bayraktar, M., Hayta, S., Parlak, S., & Gurel, A. (2015). Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots. Trees, 29(4), 999-1009.
  • Bozkurt, Y., Goker, Y., & Kurtoglu, A. (1989). Some properties of sweetgum tree. Journal of the Faculty of Forestry Istanbul University, 39(1), 43-52.
  • Brondani, G.E., Araujo, M.A.D., Alcântara, B.K.D., Carvalho, J.G.D., Gonçalves, A.N., & Almeida, M.D. (2012). In vitro organogenesis of Eucalyptus grandis: Effects of boron and calcium. Acta Scientiarum Agronomy, 34, 403-411.
  • Eaton, F.M. (1944). Deficiency, toxicity, and accumulation of boron in plants. Journal of Agricultural Research, 69(6), 237-277.
  • Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytac, Z., & Adıguzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı (Eğrelti ve Tohumlu Bitkiler) [Red data book of Turkish plants (Pteridophyta and Spermatophyta)]. Turkish Nature Conservation Association & Van 100. Yıl University.
  • Findeklee, P., & Goldbach, H.E. (1996). Rapid effects of boron deficiency on cell wall elasticity modulus in Cucurbita pepo roots. Botanica Acta, 109(6), 463-465.
  • George, E.F., Hall, M.A., & De Klerk, G.J. (Eds.). (2007). Plant propagation by tissue culture: Volume 1. The background (Vol. 1). Springer Science & Business Media.
  • Hu, H., Brown, P.H., & Labavitch, J.M. (1996). Species variability in boron requirement is correlated with cell wall pectin. Journal of Experimental Botany, 47(2), 227-232.
  • Kataeva, N.V., & Butenko, R.G. (1987). Clonal micropropagation of apple trees. Acta Horticulturae, 12, 585-588.
  • Kaya, E., Alves, A., Rodrigues, L., Jenderek, M., Hernandez-Ellis, M., Ozudogru, A., & Ellis, D. (2013) Cryopreservation of Eucalyptus genetic resources. CryoLetters, 34, 608–618.
  • Kaya, E., Balcı, M.A., Akgüller, Ö., Galatalı, S., Yeniocak, S., Mercan, T., Güldağ, S., Özkaya, D.E., Öztürk, B., Celik, O., & Aktay, İ. (2021). Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey). Acta Botanica Croatica, 80(2), 199-207.
  • Kaya, E., Galatalı, S., Guldag, S., Ozturk, B., Ceylan, M., Celık, O., & Aktay, I. (2018). Mass production of medicinal plants for obtaining secondary metabolite using liquid mediums via bioreactor systems: SETISTM and RITA®. Türk Bilimsel Derlemeler Dergisi, 11(2), 5-10.
  • Kaya, E., Souza, F., Gokdogan, EY., Ceylan, M., & Jenderek, M. (2017). Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen. Turkish Journal of Biology, 41(1), 242-248.
  • Kaya, E., Souza, F.V.D., dos Santos-Serejo, J.A., & Galatali, S. (2020). Influence of dehydration on cryopreservation of Musa spp. germplasm. Acta Botanica Croatica, 79 (2), 99-104.
  • Keren, R., & Bingham, F.T. (1958). Boron in water, soils, and plants. In Advances in Soil Science (pp. 229- 276). Springer.
  • Kıvrak, Ş., & Kıvrak, I. (2017). Assessment of phenolic profile of Turkish honeys. International Journal of Food Properties, 20(4), 864-876.
  • Kıvrak-Kıran, S., Galatalı, S., Yeniocak, S., Ozkaya, D.E., Mercan, T., Guldag, S., Celik, O., Abdul Ghafoor, N., & Kaya, E. (2021). Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.. Advances in Horticultural Science, 35(3), 285-292.
  • Lank, P., & Wahl, M. (2014). Boric Acid. In Philip, W. (Ed.), Encyclopedia of Toxicology (Third Edition, 533- 535). Academic Press.
  • Liang, G.J., Huang, G.P., & Deng L. (2011). Effect of boron, sugar, calcium and DA-6 on the growth of loquat pollen tube. Journal of Zhaoqing University, 32(2), 50-56.
  • Loomis, W.D., & Durst, R.W. (1992). Chemistry and biology of boron. BioFactors, 3(4), 229-239.
  • Lovatt, C.J., & Dugger, W.M. (1984). Boron. In Frieden, E. (Ed.), Biochemistry of the essential ultratrace elements (389-421). Plenum Press.
  • Mercan, T., Galatalı, S., Özkaya, D. E., Çelik, O. & Kaya, E. (2022). Effects of different boron salt treatments on micropropagation and genetic stability in in vitro cultures of Liquidambar orientalis Miller. Journal of Boron, 7 (4), 521-527.
  • Munns, R., & Termaat, A. (1986). Whole-plant responses to salinity. Australian Journal of Plant Physiology, 13, 143-160.
  • Murashige, T., & Skoog, F.A. (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
  • Nable, R.O., Banuelos, G.S., & Paull, J.G. (1997). Boron toxicity. Plant and Soil, 193, 181-198.
  • Ozudogru, E.A., Kaya, E., Kirdok, E., & Issever-Ozturk, S. (2011). In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cellular & Developmental Biology – Plant, 47, 309–320.
  • Ozudogru, A., da Silva, D.P.C., Kaya, E., Dradi, G., Paiva, R., & Lambardi, M. (2013) In vitro Conservation and Cryopreservation of Nandina domestica an Outdoor Ornamental Shrub. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41, 638–645.
  • Souza, F.V.D., Kaya, E., de Jesus Vieira, L., da Silva Souza, A., da Silva Carvalho, M.D.J., Santos, E.B., Alves, A.A.C., & Ellis, D. (2017) Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae, 224, 302–305.
  • Warrington, K. (1923). The effects of boric acid and borax on the bread bean and certain other plants. Annals of Botany, 37, 629-672.

Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress

Year 2023, Volume: 10 Issue: 4, 570 - 582, 01.12.2023

Muhammed Mustafa Polat Ergun Kaya İbrahim Kıvrak

https://doi.org/10.21448/ijsm.1309982

Abstract

In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.

Keywords

Boric acid Salt stress Sodium metaborate Liquidambar orientalis Phenolic compounds

References

  • Acar, M.I. (1989). Determination of composition of Liquidambar orientalis Mill. balm essential oil by analyzing it with GC-MS-DS system. Forestry Research Institute Publications, 33, 5-21.
  • Alan, M., & Kaya, Z. (2003). Oriental sweet gum Liquidambar orientalis. EUFORGEN technical guidelines. www.biodiversityinternational.org/publications
  • Alan, M., Velioglu, E., Ezen, T., Sıklar, S., & Ozturk, H. (2018). Diversity of some quantitative characters of oriental sweet gum (Liquidambar orientalis Mill.) for five-year-old seedlings. Turkish Journal of Forestry Research, 5(1), 74-81.
  • Baranek, M., Krizan, B., Ondrusikova, E., & Pidra, M. (2010). DNA-methylation changes in grapevine somaclones follow in vitro culture and thermotherapy. Plant Cell, Tissue and Organ Culture, 101(1), 11-22. https://doi.org/10.1007/s11240-009-9656-1
  • Barret, P., Brinkman, M., & Beckert, M. (2006). A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. Genome, 49(11), 1399-1407.
  • Bayraktar, M., Hayta, S., Parlak, S., & Gurel, A. (2015). Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots. Trees, 29(4), 999-1009.
  • Bozkurt, Y., Goker, Y., & Kurtoglu, A. (1989). Some properties of sweetgum tree. Journal of the Faculty of Forestry Istanbul University, 39(1), 43-52.
  • Brondani, G.E., Araujo, M.A.D., Alcântara, B.K.D., Carvalho, J.G.D., Gonçalves, A.N., & Almeida, M.D. (2012). In vitro organogenesis of Eucalyptus grandis: Effects of boron and calcium. Acta Scientiarum Agronomy, 34, 403-411.
  • Eaton, F.M. (1944). Deficiency, toxicity, and accumulation of boron in plants. Journal of Agricultural Research, 69(6), 237-277.
  • Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytac, Z., & Adıguzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı (Eğrelti ve Tohumlu Bitkiler) [Red data book of Turkish plants (Pteridophyta and Spermatophyta)]. Turkish Nature Conservation Association & Van 100. Yıl University.
  • Findeklee, P., & Goldbach, H.E. (1996). Rapid effects of boron deficiency on cell wall elasticity modulus in Cucurbita pepo roots. Botanica Acta, 109(6), 463-465.
  • George, E.F., Hall, M.A., & De Klerk, G.J. (Eds.). (2007). Plant propagation by tissue culture: Volume 1. The background (Vol. 1). Springer Science & Business Media.
  • Hu, H., Brown, P.H., & Labavitch, J.M. (1996). Species variability in boron requirement is correlated with cell wall pectin. Journal of Experimental Botany, 47(2), 227-232.
  • Kataeva, N.V., & Butenko, R.G. (1987). Clonal micropropagation of apple trees. Acta Horticulturae, 12, 585-588.
  • Kaya, E., Alves, A., Rodrigues, L., Jenderek, M., Hernandez-Ellis, M., Ozudogru, A., & Ellis, D. (2013) Cryopreservation of Eucalyptus genetic resources. CryoLetters, 34, 608–618.
  • Kaya, E., Balcı, M.A., Akgüller, Ö., Galatalı, S., Yeniocak, S., Mercan, T., Güldağ, S., Özkaya, D.E., Öztürk, B., Celik, O., & Aktay, İ. (2021). Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey). Acta Botanica Croatica, 80(2), 199-207.
  • Kaya, E., Galatalı, S., Guldag, S., Ozturk, B., Ceylan, M., Celık, O., & Aktay, I. (2018). Mass production of medicinal plants for obtaining secondary metabolite using liquid mediums via bioreactor systems: SETISTM and RITA®. Türk Bilimsel Derlemeler Dergisi, 11(2), 5-10.
  • Kaya, E., Souza, F., Gokdogan, EY., Ceylan, M., & Jenderek, M. (2017). Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen. Turkish Journal of Biology, 41(1), 242-248.
  • Kaya, E., Souza, F.V.D., dos Santos-Serejo, J.A., & Galatali, S. (2020). Influence of dehydration on cryopreservation of Musa spp. germplasm. Acta Botanica Croatica, 79 (2), 99-104.
  • Keren, R., & Bingham, F.T. (1958). Boron in water, soils, and plants. In Advances in Soil Science (pp. 229- 276). Springer.
  • Kıvrak, Ş., & Kıvrak, I. (2017). Assessment of phenolic profile of Turkish honeys. International Journal of Food Properties, 20(4), 864-876.
  • Kıvrak-Kıran, S., Galatalı, S., Yeniocak, S., Ozkaya, D.E., Mercan, T., Guldag, S., Celik, O., Abdul Ghafoor, N., & Kaya, E. (2021). Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.. Advances in Horticultural Science, 35(3), 285-292.
  • Lank, P., & Wahl, M. (2014). Boric Acid. In Philip, W. (Ed.), Encyclopedia of Toxicology (Third Edition, 533- 535). Academic Press.
  • Liang, G.J., Huang, G.P., & Deng L. (2011). Effect of boron, sugar, calcium and DA-6 on the growth of loquat pollen tube. Journal of Zhaoqing University, 32(2), 50-56.
  • Loomis, W.D., & Durst, R.W. (1992). Chemistry and biology of boron. BioFactors, 3(4), 229-239.
  • Lovatt, C.J., & Dugger, W.M. (1984). Boron. In Frieden, E. (Ed.), Biochemistry of the essential ultratrace elements (389-421). Plenum Press.
  • Mercan, T., Galatalı, S., Özkaya, D. E., Çelik, O. & Kaya, E. (2022). Effects of different boron salt treatments on micropropagation and genetic stability in in vitro cultures of Liquidambar orientalis Miller. Journal of Boron, 7 (4), 521-527.
  • Munns, R., & Termaat, A. (1986). Whole-plant responses to salinity. Australian Journal of Plant Physiology, 13, 143-160.
  • Murashige, T., & Skoog, F.A. (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
  • Nable, R.O., Banuelos, G.S., & Paull, J.G. (1997). Boron toxicity. Plant and Soil, 193, 181-198.
  • Ozudogru, E.A., Kaya, E., Kirdok, E., & Issever-Ozturk, S. (2011). In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cellular & Developmental Biology – Plant, 47, 309–320.
  • Ozudogru, A., da Silva, D.P.C., Kaya, E., Dradi, G., Paiva, R., & Lambardi, M. (2013) In vitro Conservation and Cryopreservation of Nandina domestica an Outdoor Ornamental Shrub. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41, 638–645.
  • Souza, F.V.D., Kaya, E., de Jesus Vieira, L., da Silva Souza, A., da Silva Carvalho, M.D.J., Santos, E.B., Alves, A.A.C., & Ellis, D. (2017) Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae, 224, 302–305.
  • Warrington, K. (1923). The effects of boric acid and borax on the bread bean and certain other plants. Annals of Botany, 37, 629-672.
There are 34 citations in total.

Details

Primary Language English
Subjects Natural Products and Bioactive Compounds
Journal Section Articles
Authors

Muhammed Mustafa Polat 0009-0009-4163-8530

Ergun Kaya 0000-0003-4255-3802

İbrahim Kıvrak 0000-0001-9718-8238

Early Pub Date November 11, 2023
Publication Date December 1, 2023
Submission Date June 6, 2023
Published in Issue Year 2023 Volume: 10 Issue: 4

Cite

APA Polat, M. M., Kaya, E., & Kıvrak, İ. (2023). Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress. International Journal of Secondary Metabolite, 10(4), 570-582. https://doi.org/10.21448/ijsm.1309982
International Journal of Secondary Metabolite

e-ISSN: 2148-6905