Research Article
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Combined effect of nitrogen and phosphorus on growth and biochemical composition of Tetradesmus obliquus (Turpin) M.J. Wynne

Year 2022, Volume: 9 Issue: 4, 525 - 537, 21.12.2022

Füsun Akgül Rıza Akgül

https://doi.org/10.21448/ijsm.1102592
Cited By: 1

Abstract

Microalgae have many biotechnological applications in various industries including food and feed, fertilizer, biofuel, cosmetics, pharmaceutics, and wastewater treatment. Since hey produce secondary metabolites under stress conditions such as pigments, carotenoids, hydrocarbons, and vitamins, investigating the effects of stress factors on growth parameters and biochemical composition of microalgal biomass is needed in producing bioproducts.
In this paper, the combined effects of nitrogen and phosphorus on growth and the protein/amino acid and Lipid-FAMEs profiles of microalgae Tetradesmus obliquus (MAKUMACC-037) were investigated.
Nitrogen and phosphorus deficiency reduced the algal growth. Biochemical composition was changed in a nitrogen and phosphorus dependent manner.
High concentration of protein and lipid were associated with increased nitrogen and phosphorus concentration However, the FAMEs profiles were changed depending on only the nitrogen concentration.

Keywords

Bioactive compounds FAME Lipid Nutrients Specific growth rate growth rate

Supporting Institution

Burdur MEhmet AKif Ersoy Universitesi

Project Number

0455-MP-17

References

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  • Anand. J., & Arumugam. M. (2015). Enhanced lipid accumulation and biomass yield of Scenedesmus quadricauda under nitrogen starved condition. Bioresource Technology. 188. 190–194. https://doi.org/10.1016/J.BIORTECH.2014.12.097
  • Atiku. H., Mohamed. R., Al-Gheethi. A., Wurochekke. A., & Kassim. A.H.M. (2016). Harvesting of microalgae biomass from the phytoremediation process of greywater. Environmental Science and Pollution Research. 23(24). 24624 24641. https://doi.org/10.1007/S11356-016-7456-9
  • Beuckels. A., Smolders. E., & Muylaert. K. (2015). Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Research. 77. 98–106. https://doi.org/10.1016/J.WATRES.2015.03.018
  • Bongiovani. N., Popovich. C.A., Martínez. A.M., Constenla. D., & Leonardi. P.I. (2020). Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation. Bioenergy Research. 13(2). 518–529. https://doi.org/10.1007/S12155-019-10045-2/TABLES/2
  • Boussiba. S., Fan. L., & Vonshak. A. (1992). Enhancement and determination of astaxanthin accumulation in green alga Haematococcus pluvialis. Methods in Enzymology. 213(C). 386–391. https://doi.org/10.1016/0076-6879(92)13140-S
  • Breuer. G., Lamers. P.P., Martens. D.E., Draaisma. R.B., & Wijffels. R.H. (2012). The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology. 124. 217 226. https://doi.org/10.1016/J.BIORTECH.2012.08.003
  • Bligh. E. G., & Dyer. W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 37(8). 911-917.
  • Cao. J., Yuan. H.L., Li. B.Z., & Yang. J.S. (2014). Significance evaluation of the effects of environmental factors on the lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition heterotrophic condition. Bioresource Technology. 152. 177–184. https://doi.org/10.1016/J.BIORTECH.2013.10.084
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  • Chu. F.F., Chu. P.N., Shen. X.F., Lam. P.K.S., & Zeng. R.J. (2014). Effect of phosphorus on biodiesel production from Scenedesmus obliquus under nitrogen-deficiency stress. Bioresource Technology. 152. 241–246. https://doi.org/10.1016/J.BIORTECH.2013.11.013
  • Cointet. E., Wielgosz-Collin. G., Bougaran. G., Rabesaotra. V., Gonçalves. O., & Méléder. V. (2019). Effects of light and nitrogen availability on photosynthetic efficiency and fatty acid content of three original benthic diatom strains. Plos One. 14(11). e0224701. https://doi.org/10.1371/JOURNAL.PONE.0224701
  • Courchesne. N.M.D., Parisien. A., Wang. B., & Lan. C.Q. (2009). Enhancement of lipid production using biochemical. genetic and transcription factor engineering approaches. Journal of Biotechnology. 141(12). 31 41. https://doi.org/10.1016/J.JBIOTEC.2009.02.018
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  • Godoy-Hernández. G., & Vázquez-Flota. F.A. (2006). Growth Measurements. Methods in Molecular Biology. 318. 51–58. https://doi.org/10.1385/1-59259-959-1:051
  • Hempel. N., Petrick. I., & Behrendt. F. (2012). Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. Journal of Applied Phycology. 24(6). 1407 1418. https://doi.org/10.1007/s10811-012-9795-3
  • Ho. S.H., Chan. M.C., Liu. C.C., Chen. C.Y., Lee. W.L., Lee. D.J., & Chang. J.S. (2014). Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies. Bioresource Technology. 152. 275–282. https://doi.org/10.1016/J.BIORTECH.2013.11.031
  • Hockin. N.L., Mock. T., Mulholland. F., Kopriva. S., & Malin. G. (2012). The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants. Plant Physiology. 158(1). 299–312. https://doi.org/10.1104/PP.111.184333
  • Huang. Y., Lou. C., Luo. L., & Wang. X.C. (2021). Insight into nitrogen and phosphorus coupling effects on mixotrophic Chlorella vulgaris growth under stably controlled nutrient conditions. Science of the Total Environment. 752. 141747. https://doi.org/10.1016/j.scitotenv.2020.141747
  • Imamura. S., Terashita. M., Ohnuma. M., Maruyama. S., Minoda. A., Weber. A.P.M., Inouye. T., Sekine. Y., Fujita. Y., Omata. T., & Tanaka. K. (2010). Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga cyanidioschyzon merolae: genetic evidence for nitrite reduction by a sulfite reductase-like enzyme. Plant and Cell Physiology. 51(5). 707–717. https://doi.org/10.1093/PCP/PCQ043
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Combined effect of nitrogen and phosphorus on growth and biochemical composition of Tetradesmus obliquus (Turpin) M.J. Wynne

Year 2022, Volume: 9 Issue: 4, 525 - 537, 21.12.2022

Füsun Akgül Rıza Akgül

https://doi.org/10.21448/ijsm.1102592
Cited By: 1

Abstract

Microalgae have many biotechnological applications in various industries including food and feed, fertilizer, biofuel, cosmetics, pharmaceutics, and wastewater treatment. Since hey produce secondary metabolites under stress conditions such as pigments, carotenoids, hydrocarbons, and vitamins, investigating the effects of stress factors on growth parameters and biochemical composition of microalgal biomass is needed in producing bioproducts.
In this paper, the combined effects of nitrogen and phosphorus on growth and the protein/amino acid and Lipid-FAMEs profiles of microalgae Tetradesmus obliquus (MAKUMACC-037) were investigated.
Nitrogen and phosphorus deficiency reduced the algal growth. Biochemical composition was changed in a nitrogen and phosphorus dependent manner.
High concentration of protein and lipid were associated with increased nitrogen and phosphorus concentration However, the FAMEs profiles were changed depending on only the nitrogen concentration.

Keywords

Bioactive compounds FAME Lipid Nutrients Specific growth rate growth rate

Project Number

0455-MP-17

References

  • Amaro. H.M., Guedes. A.C., & Malcata. F.X. (2011). Advances and perspectives in using microalgae to produce biodiesel. Applied Energy. 88(10). 3402 3410. https://doi.org/10.1016/J.APENERGY.2010.12.014
  • Anand. J., & Arumugam. M. (2015). Enhanced lipid accumulation and biomass yield of Scenedesmus quadricauda under nitrogen starved condition. Bioresource Technology. 188. 190–194. https://doi.org/10.1016/J.BIORTECH.2014.12.097
  • Atiku. H., Mohamed. R., Al-Gheethi. A., Wurochekke. A., & Kassim. A.H.M. (2016). Harvesting of microalgae biomass from the phytoremediation process of greywater. Environmental Science and Pollution Research. 23(24). 24624 24641. https://doi.org/10.1007/S11356-016-7456-9
  • Beuckels. A., Smolders. E., & Muylaert. K. (2015). Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Research. 77. 98–106. https://doi.org/10.1016/J.WATRES.2015.03.018
  • Bongiovani. N., Popovich. C.A., Martínez. A.M., Constenla. D., & Leonardi. P.I. (2020). Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation. Bioenergy Research. 13(2). 518–529. https://doi.org/10.1007/S12155-019-10045-2/TABLES/2
  • Boussiba. S., Fan. L., & Vonshak. A. (1992). Enhancement and determination of astaxanthin accumulation in green alga Haematococcus pluvialis. Methods in Enzymology. 213(C). 386–391. https://doi.org/10.1016/0076-6879(92)13140-S
  • Breuer. G., Lamers. P.P., Martens. D.E., Draaisma. R.B., & Wijffels. R.H. (2012). The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology. 124. 217 226. https://doi.org/10.1016/J.BIORTECH.2012.08.003
  • Bligh. E. G., & Dyer. W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 37(8). 911-917.
  • Cao. J., Yuan. H.L., Li. B.Z., & Yang. J.S. (2014). Significance evaluation of the effects of environmental factors on the lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition heterotrophic condition. Bioresource Technology. 152. 177–184. https://doi.org/10.1016/J.BIORTECH.2013.10.084
  • Chia. M.A., Lombardi. A.T., & Melão. M. da G.G. (2013). Growth and biochemical composition of Chlorella vulgaris in different growth media. Anais Da Academia Brasileira de Ciencias. 85(4). 1427–1438. https://doi.org/10.1590/0001-3765201393312
  • Chu. F.F., Chu. P.N., Shen. X.F., Lam. P.K.S., & Zeng. R.J. (2014). Effect of phosphorus on biodiesel production from Scenedesmus obliquus under nitrogen-deficiency stress. Bioresource Technology. 152. 241–246. https://doi.org/10.1016/J.BIORTECH.2013.11.013
  • Cointet. E., Wielgosz-Collin. G., Bougaran. G., Rabesaotra. V., Gonçalves. O., & Méléder. V. (2019). Effects of light and nitrogen availability on photosynthetic efficiency and fatty acid content of three original benthic diatom strains. Plos One. 14(11). e0224701. https://doi.org/10.1371/JOURNAL.PONE.0224701
  • Courchesne. N.M.D., Parisien. A., Wang. B., & Lan. C.Q. (2009). Enhancement of lipid production using biochemical. genetic and transcription factor engineering approaches. Journal of Biotechnology. 141(12). 31 41. https://doi.org/10.1016/J.JBIOTEC.2009.02.018
  • FAO. 2017. The future of food and agriculture – Trends and challenges. Rome.
  • Fernandes. B., Teixeira. J., Dragone. G., Vicente. A. A., Kawano. S., Bišová. K., Přibyl. P., Zachleder. V., & Vítová. M. (2013). Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the microalga Parachlorella kessleri. Bioresource Technology. 144. 268–274. https://doi.org/10.1016/J.BIORTECH.2013.06.096
  • Gao. B., Liu. J., Zhang. C., & Van de Waal. D.B. (2018). Biological stoichiometry of oleaginous microalgal lipid synthesis: The role of N:P supply ratios and growth rate on microalgal elemental and biochemical composition. Algal Research. 32. 353 361. https://doi.org/10.1016/J.ALGAL.2018.04.019
  • Gao. Y., Yang. M., & Wang. C. (2013). Nutrient deprivation enhances lipid content in marine microalgae. Bioresource Technology. 147. 484 491. https://doi.org/10.1016/J.BIORTECH.2013.08.066
  • Godoy-Hernández. G., & Vázquez-Flota. F.A. (2006). Growth Measurements. Methods in Molecular Biology. 318. 51–58. https://doi.org/10.1385/1-59259-959-1:051
  • Hempel. N., Petrick. I., & Behrendt. F. (2012). Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. Journal of Applied Phycology. 24(6). 1407 1418. https://doi.org/10.1007/s10811-012-9795-3
  • Ho. S.H., Chan. M.C., Liu. C.C., Chen. C.Y., Lee. W.L., Lee. D.J., & Chang. J.S. (2014). Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies. Bioresource Technology. 152. 275–282. https://doi.org/10.1016/J.BIORTECH.2013.11.031
  • Hockin. N.L., Mock. T., Mulholland. F., Kopriva. S., & Malin. G. (2012). The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants. Plant Physiology. 158(1). 299–312. https://doi.org/10.1104/PP.111.184333
  • Huang. Y., Lou. C., Luo. L., & Wang. X.C. (2021). Insight into nitrogen and phosphorus coupling effects on mixotrophic Chlorella vulgaris growth under stably controlled nutrient conditions. Science of the Total Environment. 752. 141747. https://doi.org/10.1016/j.scitotenv.2020.141747
  • Imamura. S., Terashita. M., Ohnuma. M., Maruyama. S., Minoda. A., Weber. A.P.M., Inouye. T., Sekine. Y., Fujita. Y., Omata. T., & Tanaka. K. (2010). Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga cyanidioschyzon merolae: genetic evidence for nitrite reduction by a sulfite reductase-like enzyme. Plant and Cell Physiology. 51(5). 707–717. https://doi.org/10.1093/PCP/PCQ043
  • Ji. C.F., Yu. X.J., Chen. Z.A., Xue. S., Legrand. J., & Zhang. W. (2011). Effects of nutrient deprivation on biochemical compositions and photo-hydrogen production of Tetraselmis subcordiformis. International Journal of Hydrogen Energy. 36(10). 5817–5821. https://doi.org/10.1016/J.IJHYDENE.2010.12.138
  • Ji. F., Hao. R., Liu. Y., Li. G., Zhou. Y., & Dong. R. (2013). Isolation of a novel microalgae strain Desmodesmus sp. and optimization of environmental factors for its biomass production. Bioresource Technology. 148. 249–254. https://doi.org/10.1016/J.BIORTECH.2013.08.110
  • Köse. S., Kaklikkaya. N., Koral. S., Tufan. B., Buruk. K.C., & Aydin. F. (2011). Commercial test kits and the determination of histamine in traditional (ethnic) fish products-evaluation against an EU accepted HPLC method. Food Chemistry. 125(4). 1490–1497. https://doi.org/10.1016/J.FOODCHEM.2010.10.069
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Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Füsun Akgül 0000-0002-2186-5746

Rıza Akgül 0000-0002-0280-2897

Project Number 0455-MP-17
Publication Date December 21, 2022
Submission Date April 13, 2022
Published in Issue Year 2022 Volume: 9 Issue: 4

Cite

APA Akgül, F., & Akgül, R. (2022). Combined effect of nitrogen and phosphorus on growth and biochemical composition of Tetradesmus obliquus (Turpin) M.J. Wynne. International Journal of Secondary Metabolite, 9(4), 525-537. https://doi.org/10.21448/ijsm.1102592

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e-ISSN: 2148-6905