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Effect of Ocimum basilicum on mesenchymal stem cell proliferation and differentiation: Does the effect change according to niches?

Year 2017, Volume: 4 Issue: 3, Special Issue 1, 1 - 10, 25.11.2017
https://doi.org/10.21448/ijsm.356244

Abstract

It is a big issue that reduced bone density and large fractıres in dentistry and orthopedics. Side effects caused by synthetic drugs lead to medical and ethical problems. Thus, plants and medicinal plant research take attention. Aim of this preliminary in vitro study is to investigate the effect of Ocimum basilicum extract on dental pulp (DP) and bone marrow (BM) derived mesenchymal stem cell (MSC) proliferation, osteogenic differentiation and immunological response to TNF-α. Human dental pulp tissue was obtained from patients (15-20 years of age) who were undergoing extraction of third molars for orthodontic reasons at the Department of Oral and Maxillofacial Surgery, University of Gazi University*. xCELLigence system was used to determine prolfieration of DP- and BM-MSCs. Adipogenic and osteogenic differentiation was shown and calcium concentration, osteocalcin and osteonectin levels were examined. Inflammatory environment was mimiced through TNF-α stimulation and IL-6 and IL-10 levels were defined by ELISA. Doubling time mwith O. basilicum was found in DP- MSCs (38 h) and BM-MSCs (76 h). IC50 value was shown as 148 µg/mL in DP-MSCs and 178 µg/mL in BM-MSCs. Calcium concentration of BM-MSCs was found decreased in O. basilicum treated groups. Level of ostoenectin was reduced in O. basilicum treated cells suggesting that the Extract accelerated the osteogenic differentiation. We suggest that O. basilicum could be a smart ostoeinductive agent where BM-MSCs should be investigated further. Rich flora of Turkey is an opportunity for us and encourangement can easily give inside to medicinal plant investigations. *B.30.2.GÜN.0.20-122 Ethics Committee Report

References

  • Estrela, C., Alencar, A.H.G.D., Kıtten, G.T., Vencio, E.F., Gava, E. (2011). Mesenchymal stem cells in the dent al tissues: perspectives for tissue regeneration, Brazilian Dental Journal,. 22, 91-98.
  • Gronthos, S., Ma nkani, M., Brahım, J., Robey, P.G., Shi S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo, Proceedings of the National Academic Sciences, 97, 13625-13630.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F., Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone and Mineral Research, 14, 1239-1249.
  • Akgül A. (1989). Volatile oil composition of sweet basil (Ocimum basilicum L.) cultivating in Turkey. Nahrung, 33, 87-88.
  • Baritaux, O., Richard, H., Touche, J., Derbesy, M. (1992): Effects of drying and storage of herbs and spices on the essential oil. Part I. Basil, Ocimum basilicum L., Flavour and Fragrance Journal, 7, 267-271.
  • Lahariya, A.K., & Rao, J.T. (1979). In vitro antimicrobial studies of the essential oil of Cyperus scariosus and Ocimum basilicum, Indian Drugs, 16, 150-152
  • Dube, S., Upadhyay, P.D., Tripathi, S.C. (1989). Antifungal, physicochemical and insect-repelling activity of the essential oil of Ocimum basilicum, Canadian Journal of Botany, 67, 2085-2087.
  • Özcan M. (1998). Inhibitory effects of spice extracts on the growth of Aspergillus parasiticus NRRL 2999 strain, Z. Leb.- Mitt. Unters. u- Forsch. A, 207, 253-255.
  • Martins, A.P., Salgueiro, L.R., Vila, R., Tomi, F., Canigueral, S., Casanova, J., Proença Da Cunha, A., Adzet, T. (1999). Composition of the essential oils of Ocimum canum, O. gratissimum and O. minimum, Planta Medica, 65, 187-189.
  • Godhwani, S., Godhwani, J.L., Vyas, D.S. (1987). Ocimum sanctum: An experimental study evaluating its anti-inflammatory, analgesic and antipyretic activity in animals, Journal of Ethnopharmacology, 21, 153-163.
  • Banerjee S., Prashar R., Kumar A., Rao, A.R. (1996). Modulatory influence of alcoholic extract of ocimum leaves on carcinogen-metabolizing enzyme activities and reduced glutathione levels in mouse. Nutrition and Cancer, 25, 205-217.
  • Karthikeyan, K., Ravichandran, P., Govindasamy, S. (1999). Chemopreventive effect of Ocimum sanctum on dmba-induced hamster buccal pouch carcinogenesis, Oral Oncology, 35, 12-19
  • Rastogi, S., Shukla, Y., Paul, B.N., Chowdhuri, D.K., Khanna, S.K., Das, M. (2007). Protective effect of Ocimum sanctum on 3-methylcholanthrene, 7, 12-dimethylbenz(a)anthracene and aflatoxin b1 induced skin tumorigenesis in mice, Toxicology and Applied Pharmacology, 224, 228-240.
  • Gulcin, I., Elmastas, M., Aboul-Enein, H.Y. (2007). Determination of antioxidant and radical scavenging activity of basil (ocimum basilicum l. Family lamiaceae) assayed by different methodologies, Phytotherapy Research, 21, 354-361.
  • Jayasinghe, C., Gotoh, N., Aoki, T., Wada, S. (2003). Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.), Joural of Agriculture and Food Chemistry, 51, 4442-4449.
  • Siddiqui, B.S., Aslam, H., Ali, S.T., Begum, S., Khatoon, N. (2007a). Two new triterpenoids and a steroidal glycoside from the aerial parts of Ocimum basilicum, Chemical and Pharmaceutical Bullettin (Tokyo), 55, 516-519.
  • Siddiqui, B.S., Aslam, H., Begum, S., Ali, S.T. (2007b). New cinnamic acid esters from Ocimum basilicum, Natural Product Research, 21, 736-741.
  • Zheljazkov, V.D., Callahan, A., Cantrell, C.L. (2008). Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in mississippi, Journal of Agriculture and Food Chemistry, 56, 241-245.
  • Roche Diagnostics. (2008). GmbH. Introduction of the RTCA SP Instrument. RTCA SP Instrument Operator's Manual, A. Acea Biosciences, Inc ; 14-16.
  • Pıttenger, M.F., Mackay A.M., Beck S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Hensten-Pettersen, A. (1988). Comparison of the methods available for assessing cytotoxicity. International Endodontic Journal, 21, 89-99.
  • D'aquino, R., Graziano, A., Sampaolesi, M., Laino, G., Pirozzi, G., De Rosa, A., Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation, Cell Death and Differentiation, 14, 1162-1171.
  • Dole, N.S., Kapinas, K., Kessler, C.B., Yee, S., Adams, J.D., Pereira, R.C., Delany, A.M. (2015). A single nucleotide polymorphism in osteonectin 3' untranslated region regulates bone volume and is targeted by miR-433, Journal of Bone and Mineral Research, 2015; 30, 723-32.
  • Lawrence, B.M. (1985). A review of the world production of essential oil, Perfume and Flavorance, 10, 2-16.
  • Mendi, A., Gökçınar Yağcı, B., Sarac, N., Kızıloğlu, M., Ugur, A., Yılmaz, D., Uçkan, D. (2017). The effects of Syzygium aromaticum, Cinnamomum zeylanicum and Salvia triloba extracts on proliferation and differentiation of dental pulp stem cells, Journal of Applied Oral Sciences, accepted.
  • Anpo, M., Shirayama, K., Tsutsui, T. (2011) Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic diffferentiation of human denetal pulp cells, Odontology, 99, 188-192
  • Ishihara, K., Hirano, T. (2002). IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine and Growth Factor Reviews, 13, 357-68.
  • Lieder, R., Sigurjonsson, O.E. (2014). The effect of recombinant human interleukin-6 on osteogenic differentiation and YKL-40 expression in human, bone marrow derived mesenchymal stem cells, Bioresearch Open Access, 3, 29-34.
  • Franchimont, N., Wertz, S., Malaise, M. (2005). Interleukin-6: an osteotropic factor influencing bone formation?, Bone, 37, 601-606.
  • Chipoy, C., Berreur, M., Couillaud, S., Pradal, G., Vallette, F., Colombeix, C. (2004). Downregulation of osteoblast markers and induction of the glial fibrillary acidic protein by oncostatin M in osteosarcoma cells require PKCdelta and STAT3, Journal of Bone and Mineral Research, 19, 1850-1861.
  • Malaval, L., Aubin, J.E. (2001). Biphasic effects of leukemia inhibitory factor on osteoblastic differentiation, Journal of Cell Biochemistry, 81, 63-70.
  • Malaval, L., Liu F., Vernallis A.B., Aubin J.E. (2005). GP130/OSMR is the only LIF/IL-6 family receptor complex to promote osteoblast differentiation of calvaria progenitors. Journal of Cell Physiology, 204, 585-593.
  • Falconi, D., Aubin, J.E. (2007). LIF inhibits osteoblast differentiation at least in part by regulation of HAS2 and its product hyaluronan. Journal of Bone and Mineral Research, 22, 1289-1300.

Effect of Ocimum basilicum on mesenchymal stem cell proliferation and differentiation: Does the effect change according to niches?

Year 2017, Volume: 4 Issue: 3, Special Issue 1, 1 - 10, 25.11.2017
https://doi.org/10.21448/ijsm.356244

Abstract

It is
a big issue that reduced bone density and large fractıres in dentistry and
orthopedics. Side effects caused by synthetic drugs lead to medical and ethical
problems. Thus, plants and medicinal plant research take attention. Aim of this
preliminary in vitro study is to investigate the effect of Ocimum basilicum
extract on dental pulp (DP) and bone marrow (BM) derived mesenchymal stem cell
(MSC) proliferation, osteogenic differentiation and immunological response to
TNF-α.
Human dental pulp tissue was obtained from patients
(15-20 years of age) who were undergoing extraction of third molars for
orthodontic reasons at the Department of Oral and Maxillofacial Surgery,
University of Gazi University*. xCELLigence system was used to
determine prolfieration of DP- and BM-MSCs. Adipogenic and osteogenic
differentiation was shown and calcium concentration, osteocalcin and
osteonectin levels were examined.
Inflammatory environment was mimiced through TNF-α stimulation
and IL-6 and IL-10 levels were defined by ELISA.
Doubling
time mwith O. basilicum was found in DP- MSCs (38 h) and BM-MSCs (76 h). IC50
value was shown as 148 µg/mL in DP-MSCs and 178 µg/mL in BM-MSCs. Calcium
concentration of BM-MSCs was found decreased in O. basilicum treated groups.
Level of ostoenectin was reduced in O. basilicum treated cells suggesting that
the Extract accelerated the osteogenic differentiation.
We
suggest that O. basilicum could be a smart ostoeinductive agent where BM-MSCs
should be investigated further. Rich flora of Turkey is an opportunity for us
and encourangement can easily give inside to medicinal plant investigations. *B.30.2.GÜN.0.20-122
Ethics Committee Report

References

  • Estrela, C., Alencar, A.H.G.D., Kıtten, G.T., Vencio, E.F., Gava, E. (2011). Mesenchymal stem cells in the dent al tissues: perspectives for tissue regeneration, Brazilian Dental Journal,. 22, 91-98.
  • Gronthos, S., Ma nkani, M., Brahım, J., Robey, P.G., Shi S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo, Proceedings of the National Academic Sciences, 97, 13625-13630.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F., Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone and Mineral Research, 14, 1239-1249.
  • Akgül A. (1989). Volatile oil composition of sweet basil (Ocimum basilicum L.) cultivating in Turkey. Nahrung, 33, 87-88.
  • Baritaux, O., Richard, H., Touche, J., Derbesy, M. (1992): Effects of drying and storage of herbs and spices on the essential oil. Part I. Basil, Ocimum basilicum L., Flavour and Fragrance Journal, 7, 267-271.
  • Lahariya, A.K., & Rao, J.T. (1979). In vitro antimicrobial studies of the essential oil of Cyperus scariosus and Ocimum basilicum, Indian Drugs, 16, 150-152
  • Dube, S., Upadhyay, P.D., Tripathi, S.C. (1989). Antifungal, physicochemical and insect-repelling activity of the essential oil of Ocimum basilicum, Canadian Journal of Botany, 67, 2085-2087.
  • Özcan M. (1998). Inhibitory effects of spice extracts on the growth of Aspergillus parasiticus NRRL 2999 strain, Z. Leb.- Mitt. Unters. u- Forsch. A, 207, 253-255.
  • Martins, A.P., Salgueiro, L.R., Vila, R., Tomi, F., Canigueral, S., Casanova, J., Proença Da Cunha, A., Adzet, T. (1999). Composition of the essential oils of Ocimum canum, O. gratissimum and O. minimum, Planta Medica, 65, 187-189.
  • Godhwani, S., Godhwani, J.L., Vyas, D.S. (1987). Ocimum sanctum: An experimental study evaluating its anti-inflammatory, analgesic and antipyretic activity in animals, Journal of Ethnopharmacology, 21, 153-163.
  • Banerjee S., Prashar R., Kumar A., Rao, A.R. (1996). Modulatory influence of alcoholic extract of ocimum leaves on carcinogen-metabolizing enzyme activities and reduced glutathione levels in mouse. Nutrition and Cancer, 25, 205-217.
  • Karthikeyan, K., Ravichandran, P., Govindasamy, S. (1999). Chemopreventive effect of Ocimum sanctum on dmba-induced hamster buccal pouch carcinogenesis, Oral Oncology, 35, 12-19
  • Rastogi, S., Shukla, Y., Paul, B.N., Chowdhuri, D.K., Khanna, S.K., Das, M. (2007). Protective effect of Ocimum sanctum on 3-methylcholanthrene, 7, 12-dimethylbenz(a)anthracene and aflatoxin b1 induced skin tumorigenesis in mice, Toxicology and Applied Pharmacology, 224, 228-240.
  • Gulcin, I., Elmastas, M., Aboul-Enein, H.Y. (2007). Determination of antioxidant and radical scavenging activity of basil (ocimum basilicum l. Family lamiaceae) assayed by different methodologies, Phytotherapy Research, 21, 354-361.
  • Jayasinghe, C., Gotoh, N., Aoki, T., Wada, S. (2003). Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.), Joural of Agriculture and Food Chemistry, 51, 4442-4449.
  • Siddiqui, B.S., Aslam, H., Ali, S.T., Begum, S., Khatoon, N. (2007a). Two new triterpenoids and a steroidal glycoside from the aerial parts of Ocimum basilicum, Chemical and Pharmaceutical Bullettin (Tokyo), 55, 516-519.
  • Siddiqui, B.S., Aslam, H., Begum, S., Ali, S.T. (2007b). New cinnamic acid esters from Ocimum basilicum, Natural Product Research, 21, 736-741.
  • Zheljazkov, V.D., Callahan, A., Cantrell, C.L. (2008). Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in mississippi, Journal of Agriculture and Food Chemistry, 56, 241-245.
  • Roche Diagnostics. (2008). GmbH. Introduction of the RTCA SP Instrument. RTCA SP Instrument Operator's Manual, A. Acea Biosciences, Inc ; 14-16.
  • Pıttenger, M.F., Mackay A.M., Beck S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Hensten-Pettersen, A. (1988). Comparison of the methods available for assessing cytotoxicity. International Endodontic Journal, 21, 89-99.
  • D'aquino, R., Graziano, A., Sampaolesi, M., Laino, G., Pirozzi, G., De Rosa, A., Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation, Cell Death and Differentiation, 14, 1162-1171.
  • Dole, N.S., Kapinas, K., Kessler, C.B., Yee, S., Adams, J.D., Pereira, R.C., Delany, A.M. (2015). A single nucleotide polymorphism in osteonectin 3' untranslated region regulates bone volume and is targeted by miR-433, Journal of Bone and Mineral Research, 2015; 30, 723-32.
  • Lawrence, B.M. (1985). A review of the world production of essential oil, Perfume and Flavorance, 10, 2-16.
  • Mendi, A., Gökçınar Yağcı, B., Sarac, N., Kızıloğlu, M., Ugur, A., Yılmaz, D., Uçkan, D. (2017). The effects of Syzygium aromaticum, Cinnamomum zeylanicum and Salvia triloba extracts on proliferation and differentiation of dental pulp stem cells, Journal of Applied Oral Sciences, accepted.
  • Anpo, M., Shirayama, K., Tsutsui, T. (2011) Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic diffferentiation of human denetal pulp cells, Odontology, 99, 188-192
  • Ishihara, K., Hirano, T. (2002). IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine and Growth Factor Reviews, 13, 357-68.
  • Lieder, R., Sigurjonsson, O.E. (2014). The effect of recombinant human interleukin-6 on osteogenic differentiation and YKL-40 expression in human, bone marrow derived mesenchymal stem cells, Bioresearch Open Access, 3, 29-34.
  • Franchimont, N., Wertz, S., Malaise, M. (2005). Interleukin-6: an osteotropic factor influencing bone formation?, Bone, 37, 601-606.
  • Chipoy, C., Berreur, M., Couillaud, S., Pradal, G., Vallette, F., Colombeix, C. (2004). Downregulation of osteoblast markers and induction of the glial fibrillary acidic protein by oncostatin M in osteosarcoma cells require PKCdelta and STAT3, Journal of Bone and Mineral Research, 19, 1850-1861.
  • Malaval, L., Aubin, J.E. (2001). Biphasic effects of leukemia inhibitory factor on osteoblastic differentiation, Journal of Cell Biochemistry, 81, 63-70.
  • Malaval, L., Liu F., Vernallis A.B., Aubin J.E. (2005). GP130/OSMR is the only LIF/IL-6 family receptor complex to promote osteoblast differentiation of calvaria progenitors. Journal of Cell Physiology, 204, 585-593.
  • Falconi, D., Aubin, J.E. (2007). LIF inhibits osteoblast differentiation at least in part by regulation of HAS2 and its product hyaluronan. Journal of Bone and Mineral Research, 22, 1289-1300.
There are 33 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Ayşegül H. Mendi

Beyza Gökçınar Yağcı This is me

Nurdan Sarac

Mustafa Kızıloğlu This is me

Derviş Yılmaz This is me

Duygu Uçkan This is me

Publication Date November 25, 2017
Submission Date November 19, 2017
Published in Issue Year 2017 Volume: 4 Issue: 3, Special Issue 1

Cite

APA Mendi, A. H., Gökçınar Yağcı, B., Sarac, N., Kızıloğlu, M., et al. (2017). Effect of Ocimum basilicum on mesenchymal stem cell proliferation and differentiation: Does the effect change according to niches?. International Journal of Secondary Metabolite, 4(3, Special Issue 1), 1-10. https://doi.org/10.21448/ijsm.356244
International Journal of Secondary Metabolite

e-ISSN: 2148-6905