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Farklı Hücre Dizilerinde 808 nm Laser Uygulaması ve DNA Sentezi

Year 2019, Volume: 12 Issue: 3, 1301 - 1306, 31.12.2019
https://doi.org/10.18185/erzifbed.654624

Abstract



Fotodinamik Terapi (FDT), kanser tedavisinde lokal olarak kullanılan ve
yan etkileri minimum düzeyde olan non-invazif bir yöntemdir. FDT
bileşenlerinden biri olan fotosensitizan ajan, uygun dalga boyuna sahip
ışıkla birlikte kanserli hücrelerde reaktif oksijen türlerinin ve/veya
singlet oksijen radikallerinin üretimini uyarır. Kullanılan ışık kaynağının
ve fotosensitizan ajanın tek başına bir toksik etkisinin olmadığı
bildirilmektedir. Ancak yapılan çalışmaların bir kısmında sadece laser
uygulamalarının hücre dizilerinde etkilere sahip olabileceğine dair yayınlar
da mevcuttur. Bu amaçla sıklıkla çalışılan hücre dizileri olan C6 glioma,
Caco-2 kolon kanseri, L929 fibroblast ve prostat kanseri PC-3 hücre
dizilerinde, 808 nm dalga boyuna sahip 50 J/cm
2, 100 J/cm2,
150 J/cm
2 enerji yoğunluğundaki laser ışık kaynağının 24, 48 ve 72.
saatlerdeki DNA sentezi üzerine etkileri araştırılmıştır. Laser
uygulamasının, Caco-2 hücreleri hariç, diğer tüm hücre hatlarında 24. saatte
DNA sentezini azalttığı, ancak bu etkinin diğer saatlerde kontrolden farklı
olmadığı belirlenmiştir. Sonuç olarak, uygulanan laser dozuna ve hücre
hattına bağlı olarak, laser uygulaması, kısa sürede, DNA sentezi üzerinde baskılayıcı
etkilere sahip olabilse de, bu etkilerin diğer hücresel mekanizmalar
bağlamında da araştırılması gerekmektedir.


Thanks

Boğaziçi Üniversitesi Biyomedikal Mühendisliği Enstitüsü Öğretim Üyesi Prof. Dr. Murat Gülsoy’a Biyofotonik Laboratuvarı ve Doç.Dr. Bora Garipcan’a Hücre Kültürü Laboratuvarı imkanlarından faydalanmamı sağladıkları için teşekkür ederim.

References

  • 1.Oniszczuk, A., Wojtunik-Kulesza, K.A., Oniszczuk, T., Kasprzak, K. 2016. “The potential of photodynamic therapy (PDT)—Experimental investigations and clinical use”, Biomedicine and Pharmacotherapy, 83: 912-929.
  • 2. Topaloğlu, N., Güney, M., Aysan, N.,Gülsoy, M., 2015. “The role of reactive oxygen species in the bacterial phtodynamic treatment: photoinactivation vs proliferation”, Letters in Applied Microbiology, 62(3):230-236.
  • 3. Zecha, J.A.E.M., Raber-Durlacher, J.E., Nair, R.G., Epstein, J.B., Elad, S., Hamblin, M.R., Barasch, A., Migliorati, C.A., Milstein, D.M.J., Genot, M-T., Lansaat, L., van der Brink, R., Arnabat-Dominguez, J., van der Molen, L., Jacobi, I., van Diessen, J., de Lange, J., Smeele, L.E., Schubert, M.M., Bensadoun, R-J. 2016. “Low-level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 2: proposed applications and treatment protocols” Supportive Care in Cancer, 24 (6):2781-2792.
  • 4. Yu, W., Wang, Y., Wang, J., Fang, W., Xia, K., Shao, J., Wu, M.,Liu, B., Liang, C., Ye, C., Tao, H., 2017. “A review and Outlook in the treatment osteosarcoma and other deep tumors with photodynamic therapy: from basic to deep” Oncotarget, 13 (24): 39833-39848.
  • 5. Posten, W., Wrone, D., Dover, J., Arndt, K, Silapunt, S., Alam, M., 2005. “low level Laser therapy for wound healing”, Dermatologic Surgery, 31(3):334-340.
  • 6. Schartinger, V.H., Galvan, O., Riechelmann, H., Duda, J., 2012. “Differential responses of fibroblasts, non-neoplastic epithelial cells, and oral carcinoma cells to low-level laser therapy” Supportive Care in Cancer, 20 (3): 523-529.
  • 7. AlGhamdi, K.M., Kumar, A., Moussa, N.A., 2012. “Low-level laser therapy: A useful technique for enhancing the proliferation of various cultured cells”, Lasers in Medical Science, 27 (1): 237-249.
  • 8. Xu, D., Ke, Y., Jiang, X., Cai, Y., Peng, Y., Li, Y., 2010. “In vitro photodynamic therapy on human U251 glioma cells with a novel photosensitizer ZnPcS4-BSA”. British Journal of Neurosurgery, 24, 660-665.
  • 9. Ottaviani, G., Martinelli, V., Rupel, K., Caronni, N., Naseem, A., Zandonà, L, Perinetti, G., Gobbo, M., Di Lenarda, R., Bussani, R., Benvenuti, F., Giacca, M., Biasotto, M., Zacchigna, S., 2016. “Laser Therapy Inhibits Tumor Growth in Mice by Promoting Immune Surveillance and Vessel Normalization” EBioMedicine., 11:165-172. doi: 10.1016/j.ebiom.2016.07.028.
  • 10. Dastanpour, S., Beitollahi, J.M., Saber, K., 2015. “The effect of Low-level Laser threapy on Human Leukemic Cells”, J Lasers Med Sci., 6(2): 74-79.

808 nm Laser Application and DNA Synthesis in Different Cell Lines

Year 2019, Volume: 12 Issue: 3, 1301 - 1306, 31.12.2019
https://doi.org/10.18185/erzifbed.654624

Abstract

Photodynamic
Therapy (PDT) is a non-invasive method with minimal side effects in the
treatment of cancer. The photosensitizing agent, one of the FDT components,
stimulates the generation of reactive oxygen species and / or singlet oxygen
radicals in cancer cells with light that is at appropriate wavelength. The
light source and the photosensitizing agent alone are reported to have no toxic
effect. However, in some of the studies, there are also reports that only laser
applications can have effects on cell lines. For this purpose, effects of the
808 nm laser light source at 50 J/cm2, 100 J/cm2 and 150
J/cm2 were investigated DNA synthesis of C6 glioma, Caco-2 colon
cancer, L929 fibroblast and prostate cancer PC-3 cell that are frequently
studied. It was found that except for Caco-2 cells, laser application decreased
DNA synthesis in all other cell lines at 24 hours, but this effect was maintained
at other hours. As a result, although laser application may have effects on DNA
synthesis at short time depending on the dose and cell line used, these effects
should be investigated in the context of other cellular mechanisms.

References

  • 1.Oniszczuk, A., Wojtunik-Kulesza, K.A., Oniszczuk, T., Kasprzak, K. 2016. “The potential of photodynamic therapy (PDT)—Experimental investigations and clinical use”, Biomedicine and Pharmacotherapy, 83: 912-929.
  • 2. Topaloğlu, N., Güney, M., Aysan, N.,Gülsoy, M., 2015. “The role of reactive oxygen species in the bacterial phtodynamic treatment: photoinactivation vs proliferation”, Letters in Applied Microbiology, 62(3):230-236.
  • 3. Zecha, J.A.E.M., Raber-Durlacher, J.E., Nair, R.G., Epstein, J.B., Elad, S., Hamblin, M.R., Barasch, A., Migliorati, C.A., Milstein, D.M.J., Genot, M-T., Lansaat, L., van der Brink, R., Arnabat-Dominguez, J., van der Molen, L., Jacobi, I., van Diessen, J., de Lange, J., Smeele, L.E., Schubert, M.M., Bensadoun, R-J. 2016. “Low-level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 2: proposed applications and treatment protocols” Supportive Care in Cancer, 24 (6):2781-2792.
  • 4. Yu, W., Wang, Y., Wang, J., Fang, W., Xia, K., Shao, J., Wu, M.,Liu, B., Liang, C., Ye, C., Tao, H., 2017. “A review and Outlook in the treatment osteosarcoma and other deep tumors with photodynamic therapy: from basic to deep” Oncotarget, 13 (24): 39833-39848.
  • 5. Posten, W., Wrone, D., Dover, J., Arndt, K, Silapunt, S., Alam, M., 2005. “low level Laser therapy for wound healing”, Dermatologic Surgery, 31(3):334-340.
  • 6. Schartinger, V.H., Galvan, O., Riechelmann, H., Duda, J., 2012. “Differential responses of fibroblasts, non-neoplastic epithelial cells, and oral carcinoma cells to low-level laser therapy” Supportive Care in Cancer, 20 (3): 523-529.
  • 7. AlGhamdi, K.M., Kumar, A., Moussa, N.A., 2012. “Low-level laser therapy: A useful technique for enhancing the proliferation of various cultured cells”, Lasers in Medical Science, 27 (1): 237-249.
  • 8. Xu, D., Ke, Y., Jiang, X., Cai, Y., Peng, Y., Li, Y., 2010. “In vitro photodynamic therapy on human U251 glioma cells with a novel photosensitizer ZnPcS4-BSA”. British Journal of Neurosurgery, 24, 660-665.
  • 9. Ottaviani, G., Martinelli, V., Rupel, K., Caronni, N., Naseem, A., Zandonà, L, Perinetti, G., Gobbo, M., Di Lenarda, R., Bussani, R., Benvenuti, F., Giacca, M., Biasotto, M., Zacchigna, S., 2016. “Laser Therapy Inhibits Tumor Growth in Mice by Promoting Immune Surveillance and Vessel Normalization” EBioMedicine., 11:165-172. doi: 10.1016/j.ebiom.2016.07.028.
  • 10. Dastanpour, S., Beitollahi, J.M., Saber, K., 2015. “The effect of Low-level Laser threapy on Human Leukemic Cells”, J Lasers Med Sci., 6(2): 74-79.
There are 10 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Makaleler
Authors

Ayşe Ak 0000-0002-1385-5847

Publication Date December 31, 2019
Published in Issue Year 2019 Volume: 12 Issue: 3

Cite

APA Ak, A. (2019). Farklı Hücre Dizilerinde 808 nm Laser Uygulaması ve DNA Sentezi. Erzincan University Journal of Science and Technology, 12(3), 1301-1306. https://doi.org/10.18185/erzifbed.654624