Research Article
Bioactivity of a betabaculovirus, Hyphantria cunea granulovirus, in six lepidopteran insects as potential hosts
Abstract
The aim of this study, conducted in 2018 and 2020, was to investigate the bioactivity of a local baculovirus isolate, Hyphantria cunea granulovirus (HycuGV), in seven lepidopteran pests. Based on data collected 10 days after exposure, HycuGV was found to infect Malacosoma neustria (L., 1758) (Lepidoptera: Lasiocampidae), Lymantria dispar (L., 1758) (Lepidoptera: Erebidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) and Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae) larvae as well as its host Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae). However, it did not infect Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae) and Cydia pomonella (L., 1758) (Lepidoptera: Tortricidae). A HycuGV dose rate experiment indicated LC50 of 4.7x105 occlusion bodies (OBs)/ml in H. cunea, 5.6x106 OBs/ml in L. dispar, 7x107 OBs/ml in S. exigua, 1.5x109 OBs/ml in M. neustria and 7.7x109 OBs/ml in H. armigera. HycuGV was infectious to S. exigua and L. dispar, but only provided effective control in M. neustria and H. armigera at high dose rates. These findings demonstrate that HycuGV can be highly effective for control of S. exigua, L. dispar and H. cunea.
Supporting Institution
Karadeniz Teknik Üniversitesi
Project Number
KTU BAP 5173
Thanks
This study was supported by the Karadeniz Technical University Research Foundation (grant number KTU BAP 5173).
References
- Abbott, W. S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18 (2): 265-267.
- Ahmad, I., F. Ahmad & J. Pichtel, 2011. Microbes and Microbial Technology: Agricultural and Environmental Applications. Springer, Media LLC, New York, 516 pp.
- Bayramoglu, Z., R. Nalcacioglu, Z. Demirbag & I. Demir, 2018. Characterization of a Betabaculovirus from the fall webworm, Hyphantria cunea Drury. (Lepidoptera: Erebidae), in Turkey. Biocontrol Science & Technology, 28 (12): 1178-1190.
- Brodeur, J., 2012. Host specificity in biological control: insights from opportunistic pathogens. Evolutionary Applications, 5 (5): 470-480.
- Cory, J. S., 2003. “Ecological Impacts of Virus Insecticides: Host Range and Non-Target Organisms, 73-91”. In: Environmental Impacts of Microbial Insecticides (Eds. M. T. Heikki, H. Hokkanen & A. Hajek). Springer, Dordrecht, 269 pp.
- Cory, J. S. & P. F. Entwistle, 1990. Assessing the risks of releasing genetically manipulated baculoviruses. Aspects of Applied Biology, 24 (1): 187-194.
- Cory, J. S., R. S. Hails & S. M. Sait, 1997. “Baculovirus Ecology, 301-339”. In: The Baculoviruses (Ed. L. K. Miller). Plenum, New York, 450 pp.
- Del Rincon-Castro, M. & J. E. Ibarra, 1997. Genotypic divergence of three single nuclear polyhedrosis virus (SNPV) strains from the cabbage looper, Trichoplusia ni. Biochemical Systematics and Ecology, 25 (4): 287-295.
- Federici, B. A., 1997. “Baculovirus Pathogenesis, 39-59”. In: The Baculoviruses (Ed. L. K. Miller). Plenum Press, New York, 450 pp.
- Finney, D. J., 1952. Probit Analysis: A Statistical Treatment of the Sigmoid Response Curve. Cambridge University Press, Cambridge, England, 256 pp.
- Gencer, D., Z. Bayramoglu, R. Nalcacioglu, Z. Demirbag & I. Demir, 2020. Genome sequence analysis and organization of the Hyphantria cunea granulovirus (HycuGV-Hc1) from Turkey. Genomics, 112 (1): 459-466.
- Ghulam, A., W. van der Wopke & J. M. Vlak, 2017. Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirs. Biocontrol Science and Technology, 28 (1): 20-33.
- Goulson, D., 2003. Can host susceptibility to baculovirus infection be predicted from host taxonomy or life history? Environmental Entomology, 32 (1): 61-70.
- Gröner, A., 1986. “Specifity and Safety of Baculoviruses, 177-202”. In: The Biology of Baculoviruses (Eds. R. R. Granados & B. A. Federici). CRC Press, Boca Raton, 275 pp.
- Haase, S., A. Sciocco-Cap & V. Romanowski, 2015. Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives. Viruses, 7 (5): 2230-2267.
- Hamm, J. J., 1982. Extension of the host range for a granulosis virus from Heliothis armiger from South Africa. Environmental Entomology, 11 (1): 159-160.
- Herniou, E. A. & J. A. Jehle, 2007. Baculovirus Phylogeny and Evolution. Current Drug Targets, 8 (10): 1043-1050.
- Hukuhara, T., H. Aruga & M. Kobayashi, 1969. On the Granulosis of Hyphantria cunea Drury. Japanese Journal of Applied Entomology and Zoology, 13 (1): 1-4.
- Ignoffo, C. M., 1968. Specificity of insect viruses. Bulletin of the Entomological Society of America, 14 (4): 265-276.
- Jehle, J. A., M. Lange, H. Wang, Z. Hu, Y. Wang & R. Hauschild, 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346 (1): 180-193.
- Moscardi, F., 1999. Assessment of the application of baculoviruses for control of Lepidoptera. Annual Review of Entomology, 44 (1): 257-289.
- Murphy, F. A., C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo & M. D. Summers, 1995. Virus Taxonomy: The Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses, Springer-Verlag, Wien, 586 pp.
- Opoku-Debrah, J. K., M. P. Hill, C. Knox & S. D. Moore, 2013. Overcrowding of false codling moth, Thaumatotibia leucotreta (Meyrick) leads to the isolation of five new Cryptophlebia leucotreta granulovirus (CrleGV-SA) isolates. Journal of Invertebrate Pathology, 112 (3): 219-228.
- Riwkin, H., J. S. Kroemer, A. Bronshtein, E. Belausov, B. A. Webb & N. Chejanovsky, 2006. Response of immunocompetent and immunosuppressed Spodoptera littoralis larvae to baculovirus infection. Journal of General Virology, 87 (1): 2217-2225.
- Rohrmann, G., 2013. Baculovirus Molecular Biology. Bethesda (MD), National Library of Medicine (US), National Center for Biotechnology Information, 154 pp.
- Tomita, K. O. & T. Ebihara, 1982. Cross-transmission of the Granulosis virus of the Hyphantria cunea Drury (Lepidoptera: Arctiidae), to other Lepidopterous insect’s species. Japanese Journal of Applied Entomology and Zoology, 26 (4): 224-227.
- Vasiljevic, L., 1968. Utijac virusa granuloze dudovca (Hyphantria cunea DRURY) na gusenice svilene bube (Bombyx mori L.) i kupusara (Pierris rapae L.). Plant Protection Beograd, 98 (1): 29-40 (in Croatian with abstract in English).
- Vaughn, J. L., 1974. “Virus and Richettsial Diseases, 59-62”. In: Insect Diseases (Ed. G. L. Cantwell). Marcel Dekker, New York, 595 pp.
Betabaculovirüs, Hyphantria cunea granulovirüs’ün potansiyel konukçu olarak altı lepidopter böcekteki biyoaktivitesi
Abstract
2018 ve 2020 yıllarında yürütülen bu çalışmanın amacı, yerel bir bakülovirüs izolatı Hyphantria cunea granulovirus (HycuGV)'ün yedi lepidopter zararlısı üzerindeki biyoaktivitesinin araştırılmasıdır. Denemeden sonraki 10. günde elde edilen verilere göre HycuGV’nin kendi konukçusu olan Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae)’nın yanı sıra Malacosoma neustria (L., 1758) (Lepidoptera: Lasiocampidae), Lymantria dispar (L., 1758) (Lepidoptera: Erebidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) ve Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae) larvaları üzerinde de enfeksiyon oluşturma kabiliyetine sahip olduğu belirlenirken, Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae) ve Cydia pomonella (L., 1758) (Lepidoptera: Tortricidae) larvalarında enfeksiyon oluşturmadığı tespit edildi. HycuGV’nin doz denemelerinde LC50 değeri, H. cunea’da 4.7x105 OBs/ml, L. dispar’da 5.6x106 OBs/ml, S. exigua’da 7x107 OBs/ml, M. neustria’da 1.5x109 OBs/ml ve H. armigera’da 7.7x109 OBs/ml olarak hesaplandı. Bu sonuçlar HycuGV’nin S. exigua ve L. dispar için bulaşıcı olduğunu, ancak M. neustria ve H. armigera’da ise yüksek doz oranlarında etkili olduğunu gösterdi. Bu bulgular, HycuGV’nin S. exigua, L. dispar ve H. cunea’nın mücadelesi için oldukça etkili olabileceğini göstermektedir.
Project Number
KTU BAP 5173
References
- Abbott, W. S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18 (2): 265-267.
- Ahmad, I., F. Ahmad & J. Pichtel, 2011. Microbes and Microbial Technology: Agricultural and Environmental Applications. Springer, Media LLC, New York, 516 pp.
- Bayramoglu, Z., R. Nalcacioglu, Z. Demirbag & I. Demir, 2018. Characterization of a Betabaculovirus from the fall webworm, Hyphantria cunea Drury. (Lepidoptera: Erebidae), in Turkey. Biocontrol Science & Technology, 28 (12): 1178-1190.
- Brodeur, J., 2012. Host specificity in biological control: insights from opportunistic pathogens. Evolutionary Applications, 5 (5): 470-480.
- Cory, J. S., 2003. “Ecological Impacts of Virus Insecticides: Host Range and Non-Target Organisms, 73-91”. In: Environmental Impacts of Microbial Insecticides (Eds. M. T. Heikki, H. Hokkanen & A. Hajek). Springer, Dordrecht, 269 pp.
- Cory, J. S. & P. F. Entwistle, 1990. Assessing the risks of releasing genetically manipulated baculoviruses. Aspects of Applied Biology, 24 (1): 187-194.
- Cory, J. S., R. S. Hails & S. M. Sait, 1997. “Baculovirus Ecology, 301-339”. In: The Baculoviruses (Ed. L. K. Miller). Plenum, New York, 450 pp.
- Del Rincon-Castro, M. & J. E. Ibarra, 1997. Genotypic divergence of three single nuclear polyhedrosis virus (SNPV) strains from the cabbage looper, Trichoplusia ni. Biochemical Systematics and Ecology, 25 (4): 287-295.
- Federici, B. A., 1997. “Baculovirus Pathogenesis, 39-59”. In: The Baculoviruses (Ed. L. K. Miller). Plenum Press, New York, 450 pp.
- Finney, D. J., 1952. Probit Analysis: A Statistical Treatment of the Sigmoid Response Curve. Cambridge University Press, Cambridge, England, 256 pp.
- Gencer, D., Z. Bayramoglu, R. Nalcacioglu, Z. Demirbag & I. Demir, 2020. Genome sequence analysis and organization of the Hyphantria cunea granulovirus (HycuGV-Hc1) from Turkey. Genomics, 112 (1): 459-466.
- Ghulam, A., W. van der Wopke & J. M. Vlak, 2017. Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirs. Biocontrol Science and Technology, 28 (1): 20-33.
- Goulson, D., 2003. Can host susceptibility to baculovirus infection be predicted from host taxonomy or life history? Environmental Entomology, 32 (1): 61-70.
- Gröner, A., 1986. “Specifity and Safety of Baculoviruses, 177-202”. In: The Biology of Baculoviruses (Eds. R. R. Granados & B. A. Federici). CRC Press, Boca Raton, 275 pp.
- Haase, S., A. Sciocco-Cap & V. Romanowski, 2015. Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives. Viruses, 7 (5): 2230-2267.
- Hamm, J. J., 1982. Extension of the host range for a granulosis virus from Heliothis armiger from South Africa. Environmental Entomology, 11 (1): 159-160.
- Herniou, E. A. & J. A. Jehle, 2007. Baculovirus Phylogeny and Evolution. Current Drug Targets, 8 (10): 1043-1050.
- Hukuhara, T., H. Aruga & M. Kobayashi, 1969. On the Granulosis of Hyphantria cunea Drury. Japanese Journal of Applied Entomology and Zoology, 13 (1): 1-4.
- Ignoffo, C. M., 1968. Specificity of insect viruses. Bulletin of the Entomological Society of America, 14 (4): 265-276.
- Jehle, J. A., M. Lange, H. Wang, Z. Hu, Y. Wang & R. Hauschild, 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346 (1): 180-193.
- Moscardi, F., 1999. Assessment of the application of baculoviruses for control of Lepidoptera. Annual Review of Entomology, 44 (1): 257-289.
- Murphy, F. A., C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo & M. D. Summers, 1995. Virus Taxonomy: The Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses, Springer-Verlag, Wien, 586 pp.
- Opoku-Debrah, J. K., M. P. Hill, C. Knox & S. D. Moore, 2013. Overcrowding of false codling moth, Thaumatotibia leucotreta (Meyrick) leads to the isolation of five new Cryptophlebia leucotreta granulovirus (CrleGV-SA) isolates. Journal of Invertebrate Pathology, 112 (3): 219-228.
- Riwkin, H., J. S. Kroemer, A. Bronshtein, E. Belausov, B. A. Webb & N. Chejanovsky, 2006. Response of immunocompetent and immunosuppressed Spodoptera littoralis larvae to baculovirus infection. Journal of General Virology, 87 (1): 2217-2225.
- Rohrmann, G., 2013. Baculovirus Molecular Biology. Bethesda (MD), National Library of Medicine (US), National Center for Biotechnology Information, 154 pp.
- Tomita, K. O. & T. Ebihara, 1982. Cross-transmission of the Granulosis virus of the Hyphantria cunea Drury (Lepidoptera: Arctiidae), to other Lepidopterous insect’s species. Japanese Journal of Applied Entomology and Zoology, 26 (4): 224-227.
- Vasiljevic, L., 1968. Utijac virusa granuloze dudovca (Hyphantria cunea DRURY) na gusenice svilene bube (Bombyx mori L.) i kupusara (Pierris rapae L.). Plant Protection Beograd, 98 (1): 29-40 (in Croatian with abstract in English).
- Vaughn, J. L., 1974. “Virus and Richettsial Diseases, 59-62”. In: Insect Diseases (Ed. G. L. Cantwell). Marcel Dekker, New York, 595 pp.
There are 28 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Project Number | KTU BAP 5173 |
| Early Pub Date | November 17, 2021 |
| Publication Date | December 15, 2021 |
| Submission Date | July 28, 2021 |
| Acceptance Date | November 13, 2021 |
| Published in Issue | Year 2021 Volume: 45 Issue: 4 |
