egejfasEge Journal of Fisheries and Aquatic Sciences2148-3140Ege UniversityEffects of nitrogen sources and concentration on the growth and pigment composition of the Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae).Azot Kaynakları ve Konsantrasyonlarının Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae)'nın Büyüme ve Pigment Komposizyonuna Etkisi.DurmazYaşar 09012006233112072015Azot kaynakları ve konsantrasyonlarının Nannochloropsis oculata türünün karotenoidler ve klorofil a üretimi üzerine etkileri incelendi. N. oculata, laboratuvarda 2 L’lik erlenlerde, F/2 besin ortamı ile zenginleştirilmiş steril deniz suyunda yığın kültürleri yapıldı (Tuzluluk % 2.5). Kültürler 18±1ºC sabit sıcaklıkta tutuldu. Azot kaynakları olarak sodyum nitrat, amonyum klorür ve üre olarak kullanıldı. Azot kaynakları besin ortamlarına 882 ve 441 µmol L-1 konsantrasyonlar kullanıldı. Denemeler sonunda β-Karoten, klorofil-a, klorofil-b, violaksantin, kantaksantin, astaksantin, vokeriyaksantin ve zeaksantin N.oculata’da ana pigmentler olarak TLC yöntemi ile saptandı. Toplam karotenoidler ve klorofil a düzeyleri bakımından en yüksek değerler 882 µmol L-1 NO3- -N ile hazırlanan besin ortamında ilk alınan örneklerde elde edildi (değerler sırasıyla, 4.17±0.37 mg g-1 dw ve 12.42±1.08 mg gdw ). N. oculata türü için inorganik azot kaynaklarından NO-N ve NH4+-N ile büyüme ve biyokimyasal yapısı açısından benzer sonuçlar elde edildi. Fakat üre-N’nun bu tür için uygun kaynak olmadığı tespit edildiMikroalgler Nannochloropsis oculata karoten β-karoten klorofil a ve TLC-Brown, M.R., S.W. Jeffrey, C.D. Garland, (1989), Nutritional aspects of microalgae used in mariculture: a literature review. CSIRO Mar. Lab. Rep. 205, 44.Fidalgo, J.P., A. Cid, J. Abalde, C. 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