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Akut bakır konsantrasyonlarına maruz bırakılmış pangasus balıklarında (Pangasius hypophthalmus) saptanan hematolojik ve histolojik değişimler

Year 2019, Volume: 36 Issue: 4, 387 - 396, 15.12.2019
https://doi.org/10.12714/egejfas.36.4.10

Abstract



Bu çalışmada, hematolojik ve histolojik değişiklikleri incelemek için pangasus balıkları (Pangasius hypophthalmus) 0.0, 2.5, 5.0, 10.0 mg L-1 bakır sülfat konsantrasyonlarına 48 saat süreyle maruz bırakılmıştır. Ardından her akvaryumdan dörder balık seçilmiştir. İlk 24 saatte ve ikinci 24 saatte kan örnekleri alınmıştır. Sonrasında histolojik analiz için böbrek, karaciğer, dalak ve solungaç doku örnekleri toplanmıştır. Hematokrit, hemoglobin, eritrosit sayısı, MCHC, MCH ve MCV değerleri için kan örnekleri analiz edilmiştir. Histolojik değerlendirme içinde doku örnekleri hematoksilen ve eozin boyama yöntemi kullanılarak analiz edilmiştir. Bakır konsantrasyon artışlarına bağlı olarak pangasus balıklarında hematolojik ve histolojik bazı önemli değişiklikler saptanmıştır. ≥ 2.5 mg L-1 Cu konsantrasyonlarına maruz bırakılmış pangasusların hematokrit oranında, hemoglobin miktarlarında ve eritrosit sayısında 48 saat içinde önemli artışlar olmuştur. Fakat MCHC, MCH ve MCV değerlerinde istatiksel bir farklılık gözlenmemiştir. İlk 24 saatlik süreçte ≥ 2.5 mg L-1 Cu konsantrasyonları pangasuslarının solungaçlarında ödeme, hiperplaziye, ve füzyona yol açmıştır. 48 saat sonunda ≥ 2.5 mg L-1 konsantrasyonlar solungaçlarda yoğun hiperplaziye ve kıkırdak ve epitel dokuda dejenerasyonlara neden olmuştur. Karaciğer hücrelerinde piknotik nükleus ve dejenerasyonlar, dalakta nekroz ve hemosterin kümecikleri artışı, böbrek tübüllerinde dejenerasyon ve siyah lekeler görülmüştür. Sonuç olarak ≥ 2.5 mg L-1 akut bakır konsantrasyonları pangasus balıklarında 48 saat süre içinde yüksek mortaliteye ve histolojik bozukluklara neden olmuştur.




References

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Hematological and histological alterations detected in striped catfish (Pangasius hypophthalmus) exposed to acute copper concentrations

Year 2019, Volume: 36 Issue: 4, 387 - 396, 15.12.2019
https://doi.org/10.12714/egejfas.36.4.10

Abstract



In this study, to examine hematological and histological alterations, striped catfish exposed to 0.0, 2.5, 5.0, 10.0 mg L-1 copper concentrations for 48 hours. After that, four fish were selected per aquarium. First 24 and second 24 h, blood samples were taken. And then, their gills, liver, kidney, and spleen samples were collected. The blood samples were analyzed for hematocrit, hemoglobin, number of erythrocytes, MCHC, MCH and MCV. The tissue samples stained with standart tecnique of haematoxylin and eosin for histological assessment. Copper depending on concentrations increase caused some important hematological and histological alterations on striped catfish. Number of erythroryte, hematocrit and hemoglobin of striped catfish exposed to ≥ 2.5 mg L-1 of Cu concentrations increased in 48 h. Within first 24 h ≥ 2.5 mg L-1 of Cu caused edema, hyperplasia and fusion in gills. After 48 h, ≥ 2.5 mg L-1 of Cu formed very intense hyperplasia and degeneration of cartilage and epithelium tissue in the gills. Pyknotic nuclei and degenation in liver; necrosis and increase number of hemosterin clustering in spleen; degeneration of renal tubules and black spots in kidney were seen. As a result of that, ≥ 2.5 mg L-1 of Cu caused high mortality and histological disorders within 48 h.




References

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  • Atabati, A., Keykhosravi, A., Askari-Hesni, M., Vatandost, J. & Motamedi, M. (2015). Effects of copper sulfate on gill histopathology of grass carp (Ctenopharyngodon idella). Iranian Journal of Ichthyology, 2(1), 35-42.
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  • Duman, S. & Şahan, A. (2014). Kangal (Sivas) balıklı Çermik Termal Kaplıcası ile Topardıç Deresi'nde (Sivas) yaşayan benekli sazan Cyprinion macrostomus (Heckel, 1843) da bazı hematolojik parametreler ve non-spesifik immün yanıtın belirlenmesi. Yunus Araştırma Bülteni, 4, 21-28. DOI: 10.17693/yunusae.v2014i21954.235727
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  • Figueiredo-Fernandes, A., Ferreira-Cardoso, J., Garcia-Santos, S., Monteiro, S. M., Carrola, J., Matos, P. & Fontainhas-Fernandes, A. (2007). Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper. Pesquisa Vetterinaria Brasileira, 27(3), 103-109. DOI: 10.1590/S0100-736X2007000300004
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  • Karan, V., Vitorovic, S., Tutundzic, V. & Poleksic, V. (1998). Functional enzymes activity and gill histology of carp after copper exposure and recovery. Ecotoxicology and Environmental Safety, 40, 49-55. DOI: 10.1006/eesa.1998.1641
  • Kalay, M. & Canlı, M. (2000). Elimination of essential (Cu, Zn) and non-essential (Cd, Pb) metals from tissues of a freshwater fish Tilapia zilli. Turkish Journal of Zoology. 24, 429-436.
  • Kousar, S. & Javed, M. (2012). Evaluation of acute toxicity of copper to four fresh water fish species. International Journal of Agriculture and Biology, 14(5), 801-804.
  • Kovarova, J., Kizek, R., Adam, V., Harustiakova, D., Celechovska, O. & Svobodova, Z. (2009). Effect of cadmium chloride on metallothionein levels in carp. Sensors, 9, 4789-4803. DOI: 10.3390/s90604789
  • Li, Y., Yang, H., Liu, N., Luo, J., Wang, Q. & Wang, L. (2015). Cadmium accumulation and metallothionein biosynthesis in cadmium-treated freshwater mussel Anodonta woodiana. Plos One, 10(2), 1-15. DOI: 10.1371/journal.pone.0117037
  • Linde, A.R., Sanchez-Galan, S., Valles-Mota, P. & GarciaVazquez, E. (2001). Metallothionein as bioindicator of freshwater of metal pollution: European eel and brown trout. Ecotoxicology and Environmental Safety, 49, 60- 63. DOI: 10.1006/eesa.2001.2042
  • Liu, J.-Y., Lu, T. & Zhao, N.-M. (2000). Classification and nomenclature of plant metallothionein-like proteins based on their cysteine arrangement patterns. Acta Botanica Sinica 42, 649-652.
  • Ma, W., Wang, L., He, Y. & Yan, Y. (2008). Tissue-specific cadmium and metallothionein levels in freshwater crab Sinopotamon henanense during acute exposure to waterborne cadmium. Environmental Toxicology, 23(3), 393-400. DOI: 10.1002/tox.20339
  • Maharajan, A., Rufus Kitto, M., Paruruckumani, P. S. & Ganapiriya, V. ( 2016). Histopathology biomarker responses in Asian sea bass, Lates calcarifer (Bloch) exposed to copper. The Journal of Basic and Applied Zoology, 77, 21-30. DOI: 10.1016/j.jobaz.2016.02.001
  • Marcussen, H., Holm, P. E., Ha, L. T. & Dalsgaard, A. (2007). Food safety aspects of toxic element accumulation in fish from wastewater-fed ponds in Hanoi, Vietnam. Tropical Medicine and International Health, 12, 34-39. DOI: 10.1111/j.1365-3156.2007.01939.x
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Details

Primary Language Turkish
Subjects Hydrobiology
Journal Section Articles
Authors

Semra Küçük 0000-0002-9299-5986

Publication Date December 15, 2019
Submission Date May 9, 2019
Published in Issue Year 2019Volume: 36 Issue: 4

Cite

APA Küçük, S. (2019). Akut bakır konsantrasyonlarına maruz bırakılmış pangasus balıklarında (Pangasius hypophthalmus) saptanan hematolojik ve histolojik değişimler. Ege Journal of Fisheries and Aquatic Sciences, 36(4), 387-396. https://doi.org/10.12714/egejfas.36.4.10