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Deniz Makroalgi Codium fragile (Suringar) Hariot ’in Kimyasal Bileşimi, In-Vitro Antimikrobiyal ve Antioksidan Aktivitelerinin Analizi

Year 2022, Volume: 6 Issue: 1, 94 - 104, 30.06.2022
https://doi.org/10.31594/commagene.1084336

Abstract

Sucul alanlarda yaşayan birincil üreticiler olan deniz algleri, önemleri nedeniyle birçok araştırmaya konu olmakla birlikte ilaç, kozmetik, gıda, yakıt ve tekstil endüstrilerinde önemli rol oynayan ökaryotik ve ötrofik organizmalardır. Makroalgler, potansiyel farmakolojik kullanımları olan birkaç makro besin, mikro besin ve diğer önemli biyolojik olarak aktif bileşikler (örneğin polifenoller, enzimler ve antibiyotikler) üretmesiyle bilinmektedir. Bu araştırmada, Codium fragile (Suringar) Hariot 1889’un metanol, etanol, aseton ve su ekstrelerinin kimyasal bileşimi, antimikrobiyal ve antioksidan aktiviteleri (3 yöntem ile), toplam fenolik (TPC) ve flavonoid (TFC) içeriklerini araştırmayı amaçlandı. LC-ESI-MS/MS analizleri gallik asit, 4-hidroksibenzaldehit, 4-hidroksibenzoik asit, p-kumarik asit, salisilik asit, biokanin A ve diosgenin içeren yedi bileşiğin tanımlanmasına izin verdi. Ekstrelerin TPC ve TFC değerleri sırasıyla 10,34±0,13-64,67±0,02 µg GAEs/mg ekstre ve 12,73±2,68-36,78±1,08 µg QEs/mg ekstre olarak hesaplandı. Metanol, etanol ve aseton ekstreleri gram negatif ve gram pozitif bakterilere karşı farklı seviyelerde aktivite göstermiştir (MİK: 3.125-1.562 mg/mL). Su ekstresi ABTS•+ (%70,43±14,85) ve DPPH• (%72,61±11,44) testlerine en yüksek aktiviteyi gösterirken, aseton ekstresi CUPRAC (absorbans: 0,60±0,15) testinde en yüksek aktiviteyi gösterdi. Elde ettiğimiz sonuçlar, C. fragile'in gıda koruyucuları ve diğer endüstriyel ve farmasötik alanlarda doğal bir biyoaktif madde kaynağı olarak değerlendirilebileceğini onaylamaktadır.

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü(BAP)

Project Number

20111001

Thanks

Bu çalışmamızı 20111001 nolu proje ile deteklerinden dolayı Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü(BAP)'ne teşekkür ederiz.

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Chemical Composition, In Vitro Antimicrobial and Antioxidant Activities of Marine Macroalgae Codium fragile (Suringar) Hariot

Year 2022, Volume: 6 Issue: 1, 94 - 104, 30.06.2022
https://doi.org/10.31594/commagene.1084336

Abstract

Marine algae, which are the primary producers living in aquatic areas, are the subject of many studies due to their importance as they are eukaryotic and eutrophic organisms that play a crucial role in the pharmaceutical, cosmetic, food, fuel, and textile industries. Macroalgae are known in producing several macronutrients, micronutrients, and other important biologically active compounds (e.g. polyphenols, enzymes, and antibiotics) with potential pharmacological uses. In this research, we aimed to investigate the chemical composition, antimicrobial and antioxidant activities (with three assays), total phenolic (TPC) and flavonoid (TFC) contents of the methanol, ethanol, acetone, and water extracts of Codium fragile (Suringar) Hariot. The LC-ESI-MS/MS assessment allowed the identification of seven compounds containing gallic acid, 4-hydroxybenzaldehyde, 4-hidroxybenzoic acid, p-coumaric acid, salicylic acid, biochanin A, and diosgenin. TPC and TFC of the extracts were calculated as in the range of 10.34±0.13-64.67±0.02 µg GAEs/mg extract and 12.73±2.68-36.78±1.08 µg QEs/mg extract, respectively. All extracts of C. fragile showed antimicrobial activity against all test pathogens at different levels. The methanol, ethanol, and acetone extracts showed different levels of activity against gram-negative and gram-positive bacteria (MIC: 3.125-1.562 mg/mL). The water extract showed the highest activity in ABTS•+ (70.43±14.85%) and DPPH• (72.61±11.44%) assays while the acetone extract exhibited the best activity in CUPRAC (absorbance: 0.60±0.15) assay. The results we obtained approved that C. fragile could be valued as a natural source of bioactive agents for food preservatives and in other industrial and pharmaceutical fields.

Project Number

20111001

References

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  • Arguelles, E.D.L.R., Monsalud, R.G., & Sapin, A.B., (2019b). Chemical composition and in vitro antioxidant and antibacterial activities of Sargassum vulgare C. Agardh from Lobo, Batangas, Philippines. Journal of the International Society for Southeast Asian Agricultural Sciences, 25(1), 112-122.
  • Arguelles, E.D. (2020). Evaluation of Nutritional Composition and in vitro Antioxidant and Antibacterial Activities of Codium intricatum Okamura from Ilocos Norte (Philippines). Jordan Journal of Biological Sciences, 13(3), 375-382.
  • Aşıkkutlu, B., & Okudan, E.Ş. (2021). Macro and trace element levels of macroalgae Cystoseira foeniculacea ve Gongolaria montagnei species from mediterranean region (Antalya/ Turkey). Journal of Anatolian Environmental and Animal Sciences, 6(4), 757-764. https://doi.org/10.35229/jaes.950591
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  • Demirel, Z., Yilmaz-Koz, F. F., Karabay-Yavasoglu, U. N., Ozdemir, G., & Sukatar, A. (2009). Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. Journal of the Serbian Chemical Society, 74(6), 619-628.
  • Farvin, K.H.S, & Jacobsen, C. (2013). Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. Food Chemistry, 138, 1670-1681. https://doi.org/10.1016/j.foodchem.2012.10.078.
  • Frikha, F., Kammoun, M., Hammami, N., Mchirgui, R., Belbahri, L., Gargouri, Y., Miled, N., & Ben-Rebah, F. (2011). Chemical composition and some biological activities of marine algae collected in Tunisia. Ciencias Marinas, 37 (2), 113-124.
  • Garcia-Vaquero, M., O’Doherty, J.V., Tiwari, B.K., Sweeney, T., & Rajauria, G. (2019). Enhancing the Extraction of polysaccharides and antioxidants from macroalgae using sequential hydrothermal-assisted extraction followed by ultrasound and thermal technologies. Marine Drugs, 17, 457. http://doi.org/10.3390/md17080457
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  • Heffernan, N., Smyth, T.J., Soler-Villa, A., Fitzgerald, R.J., & Brunton, N.P. (2015). Phenolic content and antioxidant activity of fractions obtained from selected Irish macroalgae species (Laminaria digitata, Fucus serratus, Gracilaria gracilis and Codium fragile). Journal of Applied Phycology, 27, 519-530. https://doi.org/10.1007/s10811-014-0291-9.
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  • Ibtissam, C., Hassane, R., Jose, M., Francisco, D.S.J., Antonio, G.V.J., Hassan, B., & Mohamed, K. (2009). Screening of antibacterial activity in marine green and brown macroalgae from the coast of Morocco. African Journal of Biotechnology, 8(7), 1258-1262.
  • Jun, J.Y., Jung, M.J., Jeong, I.H., Yamazaki, K., Kawai, Y., & Kim, B.M. (2018). Antimicrobial and antibiofilm activities of sulfated polysaccharides from marine algae against dental plaque bacteria. Marine drugs, 16(9), 301. https://doi.org/10.3390/md16090301
  • Kadam, S.U., Tiwari, B.K., & O’Donnell, C.P. (2019). Application of Novel Extraction Technologies for Bioactives from Marine Algae. Journal of Agricultural and Food Chemistry, 61, 4667−4675. https://doi.org/10.1021/jf400819p
  • Kandhasamy, M., & Arunachalam, K.D. (2008). Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India. African Journal of Biotechnology, 7(12), 1958-1961. https://doi.org/10.5897/AJB08.120
  • Keskinkaya, H.B., Gümüş, N.E., Aşıkkutlu, B., Akköz, C., Okudan, E.Ş., & Karakurt, S. (2020). Macro and trace element levels of green algae Codium fragile (Suringar) Hariot 1889 from Dardanelles (Çanakkale/Turkey). Anadolu Orman Araştırmaları Dergisi, 6(2), 55-61. https://doi.org/10.35229/jaes.809876
  • Kim, A.D., Lee, Y., Kang, S-H., Kim, G.Y., Kim, H.S. & Hyun, J.W. (2013). Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cells. Marine drugs, 11(2), 418-430. https://doi.org/10.3390/md11020418
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There are 49 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Hatice Banu Keskinkaya 0000-0002-6970-6939

Ebru Deveci 0000-0002-2597-9898

Erdoğan Güneş 0000-0003-2833-5710

Emine Şükran Okudan 0000-0001-5309-7238

Cengiz Akköz 0000-0003-3268-0189

Numan Emre Gümüş 0000-0001-8275-3871

Serdar Karakurt 0000-0002-4449-6103

Project Number 20111001
Publication Date June 30, 2022
Submission Date March 9, 2022
Acceptance Date May 30, 2022
Published in Issue Year 2022 Volume: 6 Issue: 1

Cite

APA Keskinkaya, H. B., Deveci, E., Güneş, E., Okudan, E. Ş., et al. (2022). Chemical Composition, In Vitro Antimicrobial and Antioxidant Activities of Marine Macroalgae Codium fragile (Suringar) Hariot. Commagene Journal of Biology, 6(1), 94-104. https://doi.org/10.31594/commagene.1084336