Bazı uçucu yağların P. aeruginosa PAO1 virülansında etkinliği

Ebru Önem; Hasan Cumhur Sarısu

Research Article

Bazı uçucu yağların P. aeruginosa PAO1 virülansında etkinliği

Year 2021, Volume: 4 Issue: 2, 75 - 82, 01.07.2021

Ebru Önem Hasan Cumhur Sarısu

Abstract

Amaç: Bitkisel kökenli bazı uçucu yağların antibakteriyel etkinlikleri ile P. aeruginosa’da, QS (Quorum Sensing-çevreyi algılama) sistemi üzerine inhibisyon etkilerinin araştırılması.
Gereç Yöntemler: Çalışmada, limon (Citrus limonum), bergamot (Citrus aurantium), lavanta (Lavandula angustifolia) uçucu yağlarının Pseudomona aeruginosa PAO1’de virülans faktörleri olan elastaz ve piyosiyanin pigment üretimi ile kayma hareketi ve biyofilm oluşumu üzerine inhibisyon etkileri anti-QS aktivite testleri ile gerçekleştirilmiştir. Ayrıca bazı Gram-pozitif ve Gram-negatif bakteriler üzerinde antibakteriyel etkinlikleri araştırılmıştır.
Bulgular: Sonuçta inhibisyon etkisi araştırılan her üç yağın da virülans faktörleri üzerine farklı oranlarda inhibisyon etkilerinin olduğu, en yüksek aktivitenin her üç yağ içinde biyofilm oluşumu üzerine olduğu görülmüştür. Bergamot %85 oranında en yüksek inhibisyon yeteneğine sahip iken, lavanta %76, limon ise %65 oranında etkili olmuştur (P<0,01).
Sonuç: Antibiyotik direncine karşı geliştirilen stratejiler arasında QS olarak adlandırılan bakteriler arası iletişimin inhibisyonu ve fitofarmasötiklerin kullanımı yer almakta olup, yapılan bu çalışma ile elde edilen bulgular her iki stratejinin umut vaat ettiğini desteklemektedir.

Keywords

Bakteriyel iletişim, PAO1, Antibiyofilm, uçucu yağ

References

1. Diggle S, Whiteley, M. Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology 2020; 166:30–33. doi:10.1099/mic.0.000860. PMID 31597590.
2. Høiby N, Ciofu O, Bjarnsholt T. Pseudomonas aeruginosa biofilms in cystic fibrosis. Future Microbiol 2010; 5 (11): 1663–74. doi:10.2217/fmb.10.125. PMID 21133688.
3. Lerat E, Moran, NA.The Evolutionary History of Quorum Sensing Systems in Bacteria. Mol Biol Evol 2004; 21(5): 903-913.
4. Henke JM, Bassler BL. Bacterial social engagements. Trends Cell Biol 2004; 14(11): 648-656.
5. Altınok Ö, Gürpınar Ö, Eser Ö. Bakteriyel Biyofilmler. Tıp Fakültesi Klinikleri 2018; 1(2): 45-51.
6. Hemmati F, Salehi R, Ghotaslou R, Samadi Kafil H, Hasani A, Gholizadeh P, Nouri R, Ahangarzadeh Rezaee M. Quorum Quenching: A Potential Target for Antipseudomonal Therapy. Infect Drug Resist 2020;13:2989-3005. doi:10.2147/IDR.S263196.
7. Ersöz T. Bitkisel İlaçlar ve Gıda Takviyeleri İle İlgili Genel Yaklaşım ve Sorunlar. MİSED 2012; 11-21.
8. Saab AM, Gambari R, Sacchetti G, Guerrini A, Lampronti I, Tacchini M, El Samrani A, Medawar S, Makhlouf H, Tannoury M, Abboud J, Diab-Assaf M, Kijjoa A, Tundis R, Aoun J, Efferth T. Phytochemical and pharmacological properties of essential oils from Cedrus species. Nat Prod Res 2018; 32(12):1415-1427. doi: 10.1080/14786419.2017.1346648. PMID: 28670915.
9. Ohman DE, Cryz SJ, Iglewski BH. Isolation and characterization of a P. aeruginosa PAO mutant that produces altered elastase. J Bacteriol Res 1980; 142:836-842.
10. Essar DW, Eberly L, Hadero A, Crawford I. Identification and Characterization of genes for a second anthranilate synthase in P. aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol Res 1990; 172: 884-900.
11. Kohler T, Curty LK, Barja F, Van Delden C, Pechere JC. Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili. J Bacteriol 2000; 182 (21):5990-5996.
12. O'Toole GA. Microtiter Dish Biofilm Formation Assay. J Vis Exp 2011; 47:2437.
13. Ghaderinia P, Shapouri R. Assessment of immunogenicity of alginate microparticle containing Brucella melitensis 16M oligo polysaccharide tetanus toxoid conjugate in Mouse. Banats J Biotechnol 2017; 8(16): 83–92.
14. Fernández-Sestelo M, Carrillo JM. Environmental Effects on Yield and Composition of Essential Oil in Wild Populations of Spike Lavender (Lavandula latifolia Medik.). Agriculture 2020; 10(12):626. doi:10.3390/agriculture10120626.
15. Marotta SM, Giarratana F, Parco A, et al. Evaluation of the Antibacterial Activity of Bergamot Essential Oils on Different Listeria Monocytogenes Strains. Ital J Food Saf 2016; 5(4):6176. doi:10.4081/ijfs.2016.6176.
16. Önem E, Tüzün B, Akkoç S. Anti-quorum sensing activity in Pseudomonas aeruginosa PA01 of benzimidazolium salts: electronic, spectral and structural investigations as theoretical approach, J Biomol Struct Dyn 2021; doi: 10.1080/07391102.2021.1890222.
17. Fuentes-Gutiérrez A, Curiel-Quesada E, Correa-Basurto J, Martínez-Muñoz A, Reyes-Arellano A. N-Heterocycles Scaffolds as Quorum Sensing Inhibitors. Design, Synthesis, Biological and Docking Studies. Int J Mol Sci 2020; 21(24):9512. doi:10.3390/ijms21249512.
18. Asfour HZ. Anti-Quorum Sensing Natural Compounds. J Microsc Ultrastruct 2018; 6(1):1-10. doi:10.4103/JMAU.JMAU_10_18.
19. John KMM, Bhagwat AA, Luthria DL. Swarm motility inhibitory and antioxidant activities of pomegranate peel processed under three drying conditions. Food Chem 2017; 235:145–153. doi:10.1016/j.foodchem.2017.04.143.
20. Millezi AF, Piccoli RH, Oliveira JM, Pereira MO. Anti-biofim and Antibacterial Effect of Essential Oils and Their Major Compounds. J Essent Oil-Bear Plants 2016; 19: 624-631. 10.1080/0972060X.2014.960262.
21. Kostylev M, Kima DY, Smalley, NE, Salukhea I, Greenberga EP, Dandekara AA. Evolution of the Pseudomonas aeruginosa quorumsensing hierarchy. PNAS 2019; 116:7027–7032. doi: 10.1073/pnas.1819796116.
22. Donabedian H. Quorum sensing and its relevance to infectious diseases. J Infect 2003; 46(4): 207-214. doi:/10.1053/jinf.2002.1120
23. Mecham RP. Elastin in lung development and disease pathogenesis. Matrix Biol 2018; 73:6-20. doi:10.1016/j.matbio.2018.01.005.
24. Thibodeaux BA, Caballero AR, Marquart ME, Tommassen J, O'Callaghan RJ. Corneal virulence of Pseudomonas aeruginosa elastase B and alkaline protease produced by Pseudomonas putida. Curr Eye Res 2007;32(4):373-86. doi: 10.1080/02713680701244181. PMID: 17453960.
25. George M, Pierce G, Gabriel M, Morris C, Ahearn D. Effects of quorum sensing molecules of Pseudomonas aeruginosa on organism growth, elastase B production, and primary adhesion to hydrogel contact lenses. Eye Contact Lens 2005; 31(2):54-61. doi: 10.1097/01.icl.0000146324.28865.e8. PMID: 15798474.
26. Mori M, Ikeda N, Kato Y, Minamino M, Watabe K. Inhibition of elastase activity by essential oils in vitro. J Cosmet Dermatol 2002; 1(4):183-7. doi: 10.1111/j.1473-2165.2002.00059.x. PMID: 17147537.
27. Fuqua C, Parsek MR, Greenberg EP. Regulation of gene expression by cell to-cell communication: acyl-homoserine lactone quorum sensing. Annu Rev Genet 2001; 35:439–468.
28. Husain FM, Ahmad I, Khan MS, et al. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria. Front Microbiol 2015; 6:420. DOI: 10.3389/fmicb.2015.00420.
29. Kalia M, Yadav VK, Singh PK, Sharma D, Pandey H, Narvi SS, et al. Effect of Cinnamon Oil on Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa. PLoS ONE 2015; 10(8): e0135495. doi:10.1371/journal.pone.0135495
30. Yeung AT, Torfs EC, Jamshidi F, Bains M, Wiegand I, Hancock RE, Overhage J. Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR. J Bacteriol 2009; 191(18):5592-602. doi: 10.1128/JB.00157-09.
31. Qvortrup K, Hultqvist LD, Nilsson M, Jakobsen TH, Jansen CU, Uhd J, Andersen JB, Nielsen TE, G Ml, Tolker-Nielsen T. Small Molecule Anti-biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation. Front Chem 2019; 7(742): 1-27. doi:/10.3389/fchem.2019.00742
32. Tolker-Nielsen T. Pseudomonas aeruginosa biofilm infections: from molecular biofilm biology to new treatment possibilities. APMIS Suppl 2014; 122(138):1-51. doi: 10.1111/apm.12335.
33. Jiang Q, Chen J, Yang C, Yin Y, Yao K. Quorum Sensing: A Prospective Therapeutic Target for Bacterial Diseases. Biomed Res Int 2019; 2015978. doi: 10.1155/2019/2015978.

Activities of some essential oils in P. aeruginosa PAO1 virulence

Year 2021, Volume: 4 Issue: 2, 75 - 82, 01.07.2021

Ebru Önem Hasan Cumhur Sarısu

Abstract

Objectives: Investigation antibacterial potential of some essential oils and inhibition effect in P. aeruginosa on QS (Quorum Sensing) system.
Material and Methods: In the study, the inhibition effects of essential oils of lemon (Citrus limonum), bergamot (Citrus aurantium), lavender (Lavandula angustifolia) on elastase and pyocyanine pigment production, which are virulence factors in Pseudomona aeruginosa PAO1, and swarming motility and biofilm formation were performed by anti-QS activity tests. Also antibacterial activity on some Gram-positive and Gram-negative bacteria were investigated.
Conclusion: Among the strategies developed against antibiotic resistance are the inhibition of communication between bacteria called QS and the use of phytopharmaceuticals most potetntive ones the findings obtained in this study support the promise of both strategies.
Results: As a result, it was seen that all three oils have inhibition effect on virulence factors by different inhibition rate, and the highest activity was on biofilm formation in all three oils. Bergamot had the highest inhibitory ability at 85%, while lavender was 76% and lemon was 65% effective (P <0.01).

Keywords

Bacterial communication, PAO1, Antibiofilm, essential oil

References

1. Diggle S, Whiteley, M. Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology 2020; 166:30–33. doi:10.1099/mic.0.000860. PMID 31597590.
2. Høiby N, Ciofu O, Bjarnsholt T. Pseudomonas aeruginosa biofilms in cystic fibrosis. Future Microbiol 2010; 5 (11): 1663–74. doi:10.2217/fmb.10.125. PMID 21133688.
3. Lerat E, Moran, NA.The Evolutionary History of Quorum Sensing Systems in Bacteria. Mol Biol Evol 2004; 21(5): 903-913.
4. Henke JM, Bassler BL. Bacterial social engagements. Trends Cell Biol 2004; 14(11): 648-656.
5. Altınok Ö, Gürpınar Ö, Eser Ö. Bakteriyel Biyofilmler. Tıp Fakültesi Klinikleri 2018; 1(2): 45-51.
6. Hemmati F, Salehi R, Ghotaslou R, Samadi Kafil H, Hasani A, Gholizadeh P, Nouri R, Ahangarzadeh Rezaee M. Quorum Quenching: A Potential Target for Antipseudomonal Therapy. Infect Drug Resist 2020;13:2989-3005. doi:10.2147/IDR.S263196.
7. Ersöz T. Bitkisel İlaçlar ve Gıda Takviyeleri İle İlgili Genel Yaklaşım ve Sorunlar. MİSED 2012; 11-21.
8. Saab AM, Gambari R, Sacchetti G, Guerrini A, Lampronti I, Tacchini M, El Samrani A, Medawar S, Makhlouf H, Tannoury M, Abboud J, Diab-Assaf M, Kijjoa A, Tundis R, Aoun J, Efferth T. Phytochemical and pharmacological properties of essential oils from Cedrus species. Nat Prod Res 2018; 32(12):1415-1427. doi: 10.1080/14786419.2017.1346648. PMID: 28670915.
9. Ohman DE, Cryz SJ, Iglewski BH. Isolation and characterization of a P. aeruginosa PAO mutant that produces altered elastase. J Bacteriol Res 1980; 142:836-842.
10. Essar DW, Eberly L, Hadero A, Crawford I. Identification and Characterization of genes for a second anthranilate synthase in P. aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol Res 1990; 172: 884-900.
11. Kohler T, Curty LK, Barja F, Van Delden C, Pechere JC. Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili. J Bacteriol 2000; 182 (21):5990-5996.
12. O'Toole GA. Microtiter Dish Biofilm Formation Assay. J Vis Exp 2011; 47:2437.
13. Ghaderinia P, Shapouri R. Assessment of immunogenicity of alginate microparticle containing Brucella melitensis 16M oligo polysaccharide tetanus toxoid conjugate in Mouse. Banats J Biotechnol 2017; 8(16): 83–92.
14. Fernández-Sestelo M, Carrillo JM. Environmental Effects on Yield and Composition of Essential Oil in Wild Populations of Spike Lavender (Lavandula latifolia Medik.). Agriculture 2020; 10(12):626. doi:10.3390/agriculture10120626.
15. Marotta SM, Giarratana F, Parco A, et al. Evaluation of the Antibacterial Activity of Bergamot Essential Oils on Different Listeria Monocytogenes Strains. Ital J Food Saf 2016; 5(4):6176. doi:10.4081/ijfs.2016.6176.
16. Önem E, Tüzün B, Akkoç S. Anti-quorum sensing activity in Pseudomonas aeruginosa PA01 of benzimidazolium salts: electronic, spectral and structural investigations as theoretical approach, J Biomol Struct Dyn 2021; doi: 10.1080/07391102.2021.1890222.
17. Fuentes-Gutiérrez A, Curiel-Quesada E, Correa-Basurto J, Martínez-Muñoz A, Reyes-Arellano A. N-Heterocycles Scaffolds as Quorum Sensing Inhibitors. Design, Synthesis, Biological and Docking Studies. Int J Mol Sci 2020; 21(24):9512. doi:10.3390/ijms21249512.
18. Asfour HZ. Anti-Quorum Sensing Natural Compounds. J Microsc Ultrastruct 2018; 6(1):1-10. doi:10.4103/JMAU.JMAU_10_18.
19. John KMM, Bhagwat AA, Luthria DL. Swarm motility inhibitory and antioxidant activities of pomegranate peel processed under three drying conditions. Food Chem 2017; 235:145–153. doi:10.1016/j.foodchem.2017.04.143.
20. Millezi AF, Piccoli RH, Oliveira JM, Pereira MO. Anti-biofim and Antibacterial Effect of Essential Oils and Their Major Compounds. J Essent Oil-Bear Plants 2016; 19: 624-631. 10.1080/0972060X.2014.960262.
21. Kostylev M, Kima DY, Smalley, NE, Salukhea I, Greenberga EP, Dandekara AA. Evolution of the Pseudomonas aeruginosa quorumsensing hierarchy. PNAS 2019; 116:7027–7032. doi: 10.1073/pnas.1819796116.
22. Donabedian H. Quorum sensing and its relevance to infectious diseases. J Infect 2003; 46(4): 207-214. doi:/10.1053/jinf.2002.1120
23. Mecham RP. Elastin in lung development and disease pathogenesis. Matrix Biol 2018; 73:6-20. doi:10.1016/j.matbio.2018.01.005.
24. Thibodeaux BA, Caballero AR, Marquart ME, Tommassen J, O'Callaghan RJ. Corneal virulence of Pseudomonas aeruginosa elastase B and alkaline protease produced by Pseudomonas putida. Curr Eye Res 2007;32(4):373-86. doi: 10.1080/02713680701244181. PMID: 17453960.
25. George M, Pierce G, Gabriel M, Morris C, Ahearn D. Effects of quorum sensing molecules of Pseudomonas aeruginosa on organism growth, elastase B production, and primary adhesion to hydrogel contact lenses. Eye Contact Lens 2005; 31(2):54-61. doi: 10.1097/01.icl.0000146324.28865.e8. PMID: 15798474.
26. Mori M, Ikeda N, Kato Y, Minamino M, Watabe K. Inhibition of elastase activity by essential oils in vitro. J Cosmet Dermatol 2002; 1(4):183-7. doi: 10.1111/j.1473-2165.2002.00059.x. PMID: 17147537.
27. Fuqua C, Parsek MR, Greenberg EP. Regulation of gene expression by cell to-cell communication: acyl-homoserine lactone quorum sensing. Annu Rev Genet 2001; 35:439–468.
28. Husain FM, Ahmad I, Khan MS, et al. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria. Front Microbiol 2015; 6:420. DOI: 10.3389/fmicb.2015.00420.
29. Kalia M, Yadav VK, Singh PK, Sharma D, Pandey H, Narvi SS, et al. Effect of Cinnamon Oil on Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa. PLoS ONE 2015; 10(8): e0135495. doi:10.1371/journal.pone.0135495
30. Yeung AT, Torfs EC, Jamshidi F, Bains M, Wiegand I, Hancock RE, Overhage J. Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR. J Bacteriol 2009; 191(18):5592-602. doi: 10.1128/JB.00157-09.
31. Qvortrup K, Hultqvist LD, Nilsson M, Jakobsen TH, Jansen CU, Uhd J, Andersen JB, Nielsen TE, G Ml, Tolker-Nielsen T. Small Molecule Anti-biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation. Front Chem 2019; 7(742): 1-27. doi:/10.3389/fchem.2019.00742
32. Tolker-Nielsen T. Pseudomonas aeruginosa biofilm infections: from molecular biofilm biology to new treatment possibilities. APMIS Suppl 2014; 122(138):1-51. doi: 10.1111/apm.12335.
33. Jiang Q, Chen J, Yang C, Yin Y, Yao K. Quorum Sensing: A Prospective Therapeutic Target for Bacterial Diseases. Biomed Res Int 2019; 2015978. doi: 10.1155/2019/2015978.

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Details

Primary Language	Turkish
Subjects	Clinical Sciences
Journal Section	Research article
Authors	Ebru Önem 0000-0002-7770-7958 Hasan Cumhur Sarısu 0000-0002-4355-966X
Publication Date	July 1, 2021
Acceptance Date	June 14, 2021
Published in Issue	Year 2021 Volume: 4 Issue: 2

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APA	Önem, E., & Sarısu, H. C. (2021). Bazı uçucu yağların P. aeruginosa PAO1 virülansında etkinliği. Tıp Fakültesi Klinikleri Dergisi, 4(2), 75-82.

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