Phenolic and flavonoid amounts and antioxidant capacity of Lavandula officinalis (lavender) callus grown in different growth regulator combinations
Abstract
In this study, it was aimed to determine the total antioxidant capacity and phenolic and flavonoid amounts of Lavandula officinalis (lavender), which is an aromatic plant, callus extracts. Combinations of Naphthalene Acetic Acid (NAA), Benzylaminopurine (BAP), Kinetin (KIN) and 2,4-Dichlorophenoxy Acetic Acid (2,4-D) plant growth regulators at different concentrations were used in the growth medium. The specified analyzes were carried out for the extracts obtained from the callus of plants grown in different media. The callus were extracted with water and ethanol solvents. Total phenolic amount analyzes were accomplished using the Folin-Ciocalteu method. The spectrophotometric AlCl3 method was used for the total flavonoid amount analysis and the antioxidant capacity of lavender callus extracts was measured considering the radical cation capture ability of 2,2′-azino-bis -3- ethylbenzthiazoline -6- sulphonicacid (ABTS). The highest antioxidant capacity (9.24 ± 0.14 mmol TEAC/g dry callus weight) was obtained from the callus of plants grown in medium containing 0.5 mg/L BAP + 0.5 mg/L 2,4-D combination; the highest amount of phenolic substance (35.74± 0.48 mg GAE/g dry callus weight) was obtained from the callus of plants grown in medium containing the combination of 0.5 mg/L BAP + 0.5 mg/L NAA; the highest amount of flavonoid substance (32.42 ± 0.46 mg QE/g dry callus weight) was obtained from the callus of plants grown in medium containing the combination of 0.5 mg/L BAP + 1 mg/L 2,4-D. The results are compared for the combination of plant growth regulators and the effects of the different growth medium ingredients were specified.
Keywords
Antioxidant Callus Flavonoid Plant Growth Regulator Lavandula officinalis Phenolic. Antioksidan kallus flavonoid bitki büyüme düzenleyici fenolik
References
- El Sherif F, Albotnoor N, Yap YK, Meligy A, Khattab S (2020). Enhanced bioactive compounds composition in Lavandula officinalis in-vitro plantlets using NaCl and Moringa oleifera, Aloe vera and Spirulina platensis extracts. Industrial Crops and Products 157: 112890.
- Genç L, Kaçar O (2012). Tıbbi ve aromatik bitkilerin tarım ve hayvancılık alanlarında kullanımı. In: Kınay A, Özyılmaz B (eds). Birinci Tıbbi Aromatik Bitkiler Sempozyumu Bildirileri Kitabı Tokat, Türkiye. Pp. 7-28.
- Grzegorczyk I, Matkowski A, Wysokinska H (2007). Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chemistry 104(2): 536-541.
- Hickey M, King C (1997). Common Families of Flowering Plants. Cambridge: Cambridge Univ. Press.
- Kim HY, Kim OH, Sung MK (2003). Effects of phenol-depleted and phenol-rich diets on blood markers of oxidative stress, and urinary excretion of quercetin and kaempferol in healthy volunteers. Journal of the American College of Nutrition 22(3): 217-223.
- Koç H (2002). Bitkilerle Sağlıklı Yaşama. Ankara: T.C. Kültür Bakanlığı Kültür Eserleri Dizisi, No: 373.
- Kousalya L, Bai VN (2016). Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult. A threatened medicinal plant. Asian Pacific Journal of Reproduction 5:(2), 161-170.
- Kovacheva E, Georgiev M, Pashova S, Angelova M, Ilieva M (2006). Radical quenching by rosmarinic acid from Lavandula vera MM cell culture. Zeitschrift fur Naturforschung C - Journal of Biosciences 61(7-8): 517-520.
- Lamaison JLC, Carnet A (1990). Teneurs en principaux flavonoides des fleurs de Crataegus monogyna Jacq et de Crataegus laevigata (Poiret D. C) en fonction de la vegetation. Pharmaceutica Acta Helvetia, 65: 315-320.
- Meriç S, Tuman CB, Ayan A, Atak Ç (2019). Optimization of tissue culture media-inducing essential oil production of levander (Lavandula angustifolia Mill.). Physiology and Molecular Biology of Plants 25(1): 85-99.
- Miller NJ, Diplock AT, Rice-Evans CA (1995). Evaluation of the total antioxidant activity as a marker of the deterioration of apple juice on storage. Journal of Agricultural and Food Chemistry 43(7): 1794-1801.
- Moon T, Wilkinson JM, Cavanagh HM (2006). Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitology Research 99(6): 722-728.
- Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3): 144-158.
- Tanrıseven A (2013). Tıbbi aromatik bitkiler biyo sağlık ve ekonomi. Batı Akdeniz Tarımsal Araştırma Enstitüsü Dergisi 3: 44-58.
- Topdemir A, Gür N, Demir Z (2018). Melissa officinalis L. kallus kültürlerinin antoksidan özellikleri. Eurasian Journal of Biological and Chemical Sciences 1(1): 12-15.
- Topdemir A, Buran A, Gür N (2019a). Ocimum basilicum L. (Fesleğen) kallus kültürlerinin antioksidan kapasitesinin belirlenmesi. In: Doğan M & Atay E (eds.). 2nd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, Proceeding Book Pp. 920-928.
- Topdemir A, Buran A, Gür N (2019b). Fesleğen (Ocimum basilicum L.) bitkisi kallus kültürlerinin toplam fenolik ve flavonoid miktarının tespiti. In: Doğan M, Atay E (eds.). 2nd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, Proceeding Book. Pp. 929-936.
- Yeşil-Çeliktaş O, Nartop P, Gürel A, Bedir E, Vardar-Sukan F (2007). Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis L. Journal of Plant Physiology 164(11): 1536-1542.
- Yoon JH, Jong-II L, Chi-Kun O, Zhi C, Seung-Yul Y, Won-Seob S (2006). Effect of Plant Growth Regulator on Antioxidant Activity of Rosmarinus officinalis L. and Lavandula spica L. Korean Journal of Plant Resources 19(2): 315-322.
Farklı büyüme düzenleyici kombinasyonlarda yetiştirilen Lavandula officinalis (lavanta) kalluslarının fenolik ve flavonoid miktarları ve antioksidan kapasitesi
Abstract
Bu çalışmada, tıbbi ve aromatik bir bitki olan Lavandula officinalis (lavanta) kallus ekstraktlarının toplam antioksidan kapasitesinin ve fenolik ve flavonoid madde miktarlarının belirlenmesi amaçlanmıştır. Büyüme ortamında Naftalin Asetik Asit (NAA), Benzilaminopurin (BAP), Kinetin (KIN) ve 2,4-Diklorofenoksi Asetik Asit (2,4-D) bitki büyüme düzenleyicilerinin farklı konsantrasyonlardaki kombinasyonları kullanılmıştır. Farklı ortamlarda yetiştirilen bitkilerin kalluslarından elde edilen ekstraktlar için belirtilen analizler yapılmıştır. Kalluslar su ve etanol solventleri ile ekstrakte edilmiştir. Toplam fenolik miktarı analizleri Folin-Ciocalteu yöntemi kullanılarak yapılmıştır. Toplam flavonoid miktarı analizi için spektrofotometrik AlCl3 yöntemi kullanılmış ve lavanta kallus ekstraktlarının antioksidan kapasitesi 2,2′-azino-bis -3-etilbenztiazolin -6- sülfonik asidin (ABTS) radikal katyon yakalama kabiliyeti dikkate alınarak ölçülmüştür. En yüksek antioksidan kapasite (9,24 ± 0,14 mmol TEAC/g kuru kallus ağırlığı) 0,5 mg/L BAP + 0,5 mg/L 2,4-D kombinasyonunu içeren ortamda yetiştirilen bitkilerin kalluslarından elde edilmiştir; en yüksek fenolik madde miktarı (35,74± 0,48 mg GAE/g kuru kallus ağırlığı) 0,5 mg/L BAP + 0,5 mg/L NAA kombinasyonunu içeren ortamda yetiştirilen bitkilerin kalluslarından; en yüksek flavonoid madde miktarı (32,42 ± 0,46 mg QE/g kuru kallus ağırlığı) 0,5 mg/L BAP + 1 mg/L 2,4-D kombinasyonunu içeren ortamda yetiştirilen bitkilerin kalluslarından elde edilmiştir. Sonuçlar, bitki büyüme düzenleyicilerinin kombinasyonu için karşılaştırılmış ve farklı yetiştirme ortamı bileşenlerinin etkileri belirlenmiştir.
References
- El Sherif F, Albotnoor N, Yap YK, Meligy A, Khattab S (2020). Enhanced bioactive compounds composition in Lavandula officinalis in-vitro plantlets using NaCl and Moringa oleifera, Aloe vera and Spirulina platensis extracts. Industrial Crops and Products 157: 112890.
- Genç L, Kaçar O (2012). Tıbbi ve aromatik bitkilerin tarım ve hayvancılık alanlarında kullanımı. In: Kınay A, Özyılmaz B (eds). Birinci Tıbbi Aromatik Bitkiler Sempozyumu Bildirileri Kitabı Tokat, Türkiye. Pp. 7-28.
- Grzegorczyk I, Matkowski A, Wysokinska H (2007). Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chemistry 104(2): 536-541.
- Hickey M, King C (1997). Common Families of Flowering Plants. Cambridge: Cambridge Univ. Press.
- Kim HY, Kim OH, Sung MK (2003). Effects of phenol-depleted and phenol-rich diets on blood markers of oxidative stress, and urinary excretion of quercetin and kaempferol in healthy volunteers. Journal of the American College of Nutrition 22(3): 217-223.
- Koç H (2002). Bitkilerle Sağlıklı Yaşama. Ankara: T.C. Kültür Bakanlığı Kültür Eserleri Dizisi, No: 373.
- Kousalya L, Bai VN (2016). Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult. A threatened medicinal plant. Asian Pacific Journal of Reproduction 5:(2), 161-170.
- Kovacheva E, Georgiev M, Pashova S, Angelova M, Ilieva M (2006). Radical quenching by rosmarinic acid from Lavandula vera MM cell culture. Zeitschrift fur Naturforschung C - Journal of Biosciences 61(7-8): 517-520.
- Lamaison JLC, Carnet A (1990). Teneurs en principaux flavonoides des fleurs de Crataegus monogyna Jacq et de Crataegus laevigata (Poiret D. C) en fonction de la vegetation. Pharmaceutica Acta Helvetia, 65: 315-320.
- Meriç S, Tuman CB, Ayan A, Atak Ç (2019). Optimization of tissue culture media-inducing essential oil production of levander (Lavandula angustifolia Mill.). Physiology and Molecular Biology of Plants 25(1): 85-99.
- Miller NJ, Diplock AT, Rice-Evans CA (1995). Evaluation of the total antioxidant activity as a marker of the deterioration of apple juice on storage. Journal of Agricultural and Food Chemistry 43(7): 1794-1801.
- Moon T, Wilkinson JM, Cavanagh HM (2006). Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitology Research 99(6): 722-728.
- Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3): 144-158.
- Tanrıseven A (2013). Tıbbi aromatik bitkiler biyo sağlık ve ekonomi. Batı Akdeniz Tarımsal Araştırma Enstitüsü Dergisi 3: 44-58.
- Topdemir A, Gür N, Demir Z (2018). Melissa officinalis L. kallus kültürlerinin antoksidan özellikleri. Eurasian Journal of Biological and Chemical Sciences 1(1): 12-15.
- Topdemir A, Buran A, Gür N (2019a). Ocimum basilicum L. (Fesleğen) kallus kültürlerinin antioksidan kapasitesinin belirlenmesi. In: Doğan M & Atay E (eds.). 2nd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, Proceeding Book Pp. 920-928.
- Topdemir A, Buran A, Gür N (2019b). Fesleğen (Ocimum basilicum L.) bitkisi kallus kültürlerinin toplam fenolik ve flavonoid miktarının tespiti. In: Doğan M, Atay E (eds.). 2nd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, Proceeding Book. Pp. 929-936.
- Yeşil-Çeliktaş O, Nartop P, Gürel A, Bedir E, Vardar-Sukan F (2007). Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis L. Journal of Plant Physiology 164(11): 1536-1542.
- Yoon JH, Jong-II L, Chi-Kun O, Zhi C, Seung-Yul Y, Won-Seob S (2006). Effect of Plant Growth Regulator on Antioxidant Activity of Rosmarinus officinalis L. and Lavandula spica L. Korean Journal of Plant Resources 19(2): 315-322.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Articles |
| Authors | |
| Publication Date | November 15, 2022 |
| Acceptance Date | November 12, 2022 |
| Published in Issue | Year 2022 Volume: 6 Issue: 2 |
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Anatolian Journal of Botany is licensed under CC BY 4.0
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