Research Article
Chromomycin A3 and DAPI Staining of Chromosomes of Three Endemic Pseudophoxinus Bleeker, 1860 (Teleostei: Leuciscidae) Species from Anatolia
Abstract
The karyotypes and other chromosomal markers of representatives of the genus Pseudophoxinus have been little studied. Therefore, this study documents chromosomal NOR phenotypes as revealed using Chromomycin A3 (CMA3) and DAPI chromosomal stainings in three species Pseudophoxinus elizavetae Bogutskaya, Küçük & Atalay, 2007, P. firati Bogutskaya, Küçük & Atalay, 2007 and P. hittitorum Freyhof & Özuluğ, 2010, endemic in Anatolia. Simple NOR phenotype with only two CMA3 positive signals were observed in the karyotype of P. firati whereas derived NOR phenotype with four CMA3 positive signals were observed in those of P. elizavetae and P. hittitorum, respectively. No DAPI positive signal was detected in karyotypes in all three species. This study described new NOR phenotypes, i.e. number and position of major rDNA genes, in genomes of three Pseudophoxinus species contributing thus to known diversity of NOR phenotypes in otherwise karyotypically highly conservative leuciscid fishes.
Supporting Institution
Kırşehir Ahi Evran University
Project Number
SYO.A4.18.001
Thanks
This study was supported by Kırşehir Ahi Evran University Scientific Research Projects (Project code: SYO.A4.18.001).
References
- Anjum, R., & Jankun, M. (1998). NOR-bearing chromosomal associations revealed through silver and sequential chromomycin A3 staining in the mirror carp, Cyprinus carpio. Caryologia, 51(2), 167-171.
- Ayata, M.K., Yüksel, E., & Gaffaroğlu, M. (2016). Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae). Caryologia, 69(3), 215-222. http://doi: 10.1080/00087114.2016.1169089.
- Bianco, P.G, Aprea, G., Balletto, E., Capriglione, T., Fulgione, D., & Odierna, G. (2004). The karyology of the cyprinid genera Scardinius and Rutilus in southern Europe. Ichthyological Research, 51, 274-278.
- Boron, A. (1999). Banded karyotype of spined loach Cobitis taenia and triploid Cobitis from Poland. Genetica, 105, 293-300.
- Boron, A., Porycka, K., Ito, D., Abe, S., & Kirtiklis, L. (2009). Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, Cyprinidae) using chromosome banding and FISH with rDNA. Genetica, 135, 199-207.
- Collares-Pereira, M.J. (1992). In vivo direct chromosome preparation (Air Drying Technique). 1st International Workshop on Fish Cytogenetic Techniques, Concarneau, pp. 15-20.
- Ergene, S., Karahan, A., & Kuru, M. (2010). Cytogenetic analysis of Pseudophoxinus antalyae, Bogustkaya, 1992 (Pisces: Cyprinidae) from the Eastern Mediterranean River Basin. Turkey. Turkish Journal of Zoology, 34(1), 111-117.
- Gaffaroğlu, M., Ayata, M.K., Ünal, S. & Yüksel, E. (2014). Chromosomal analysis of Pseudophoxinus elizavetae Bogutskaya, Kucuk and Atalay, 2007 (Teleostei, Cyprinidae) from Anatolia. FABA2014: International Symposium on Fisheries and Aquatic Science; 25-27 September; Trabzon.
- Gromicho, M. & Collares-Pereira, M.J. (2004). Polymorphism of major ribosomal gene chromosomal sites (NORphenotypes) in the hybridogenetic fish Squalius alburnoides complex (Cyprinidae) assessed through crossing experiments. Genetica, 122, 291-302.
- Güçlü, S.S., & Küçük, F. (2017). Endemic species of the genus Pseudophoxinus (Teleostei: Cyprinidae) in Anatolia and distribution areas. First International Symposium on Limnology and Freshwater Fisheries. 4-6 October, 2017, Eğirdir, Turkey.
- Kapuscinski, J. (1995). DAPI: a DNA-specific fluorescent probe. Biotechnic and Histochemistry, 70(5), 220-33.
- Karasu, M. (2009). Pseudophoxinus firati (Pisces:Cyprinidae)’nin karyotip özellikleri. Gazi Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Ankara.
- Karasu, M., Yüksel, E., & Gaffaroğlu, M. (2011). Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük, & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Turkey. Turkish Journal of Zoology, 35(6), 865-868. http://doi:10.3906/zoo-0912-129.
- Kirtiklis, L., Porycka, K., Boroñ, A., Coutanceau, J.P., & Dettai, A. (2010). Use of the chromosomal co-location of the minor 5S and the major 28S rDNA as a cytogenetic marker within the genus Leuciscus (Pisces, Cyprinidae). Folia biologica (Kraków), 58, 3-4. doi:10.3409/fb58_3-4.245-249.
- Mani, I., Kumar, R., Singh, M., Kushwaha, B., Nagpure, N.S., Srivastava, P.K., Murmu, K., Rao, D.S.K., & Lakra, W.S. (2009). Karyotypic diversity and evolution of seven mahseer species (Cyprinidae) from India. Journal of Fish Biology, 75, 1079-1091
- Mayr, B., Rab, P., & Kalat, M. (1986). NORs and counterstain-enhanced fluorescence studies in Cyprinidae of different ploidy level. Genetica, 69(2), 111-118.
- Monteiro, R., Carvalho, C., & Collares-Pereira, M.J. (2009). Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister species I. lusitanicum. Genetics and Molecular Biology, 32(2), 268-275.
- Nabais, C., Rampin, M., & Collares-Pereira, M.J. (2013). Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis and S. torgalensis (Teleostei, Cyprinidae), from the Iberian Peninsula. Comparative Cytogenetics, 7(1), 33-42.
- Rab, P., Rabova, M., Economidis, P.S., & Triantaphyllidis, C. (2000). Banded karyotype of the Greek endemic cyprinid fish. Pachychilon macedonicum. Ichthyological Reserarch, 47(1), 107-110.
- Rab, P., Karakousis, M.Y., Rabova, M., & Economidis, P.S. 1996. Banded karyotype of the cyprinid fish Leuciscus borysthenicus. Ichthyological Research, 43(4), 463-468.
- Rabova, M., Rab, P., Ozouf-Costaz, C., Ene, C., & Wanzebock, J. (2003). Comparative cytogenetics and chromosomal characteristics of ribosomal DNA in the fish genus Vimba (Cyprinidae). Genetica, 118, 83-91.
- Saç, G., Özuluğ, M., Elp, M., Gaffaroğlu, M., Ünal, S., Karasu Ayata, M., Kaya, C., & Freyhof, J. (2019). New records of Pseudophoxinus firati from Turkey (Teleostei: Leuciscidae). Journal of Applied Ichthyology, 1, 1-6. https://doi. org/10.1111/jai.13869.
- Sola, L., Rossi, A.R., Iaselli, V., Rasch, E.M., & Monaco, P.J. (1992). Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana mexicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenetics and Cell Genetics, 60, 229-235. doi: 10.1159/000133346.
- Unal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369.
- Ünal, S., Gaffaroğlu, M., Ayata, M.K., & Yüksel, E. (2014). Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae). Comparative Cytogenetics, 8(4), 249-257. http://doi: 10.3897/CompCytogen.v8i4.7623.
- Valic, D., Kapetanovıć, D., Zanella, D., Mrakovčıć, M., Teskeredžıć, E., Besendorfer, V., Rabova, M., & Rab, P. (2010). The karyotype and NOR phenotype of Telestes ukliva (Cyprinidae). Folia Zoologica, 59(2), 169-173.
Anadolu’da Yaşayan Endemik Üç Pseudophoxinus Bleeker, 1860 (Teleostei: Leuciscidae) Türünün CMA3 ve DAPI Boyalı Kromozomları
Abstract
Pseudophoxinus cinsindeki balıkların kromozom ve diğer sitotaksonomik markerları konusundaki çalışma sayısı azdır. Bu çalışmada, Anadolu’ya endemik üç leuciscine türü Pseudophoxinus elizavetae Bogutskaya, Küçük & Atalay, 2007, P. firati Bogutskaya, Küçük & Atalay, 2007 ve P. hittitorum Freyhof & Özuluğ, 2010’da kromozomal NOR fenotipleri CMA3 ve DAPI kromozomal boyama ile çalışılmıştır. P. firati’nin karyotipinde iki, P. elizavetae ve P. hittitorum’un karyotiplerinde ise dört CMA3 pozitif sinyal gözlenmiştir. Üç türün karyotipinde de DAPI pozitif sinyal gözlenmemiştir. Bu çalışma üç Pseudophoxinus türünün genomunda majör rDNA genlerinin sayısı ve pozisyonu gibi yeni NOR fenotiplerini tanımlamıştır. Böylece karyotipik olarak yüksek derecede konservatif bilinen leuciscid NOR fenotip çeşitliliğine katkıda bulunur.
Project Number
SYO.A4.18.001
References
- Anjum, R., & Jankun, M. (1998). NOR-bearing chromosomal associations revealed through silver and sequential chromomycin A3 staining in the mirror carp, Cyprinus carpio. Caryologia, 51(2), 167-171.
- Ayata, M.K., Yüksel, E., & Gaffaroğlu, M. (2016). Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae). Caryologia, 69(3), 215-222. http://doi: 10.1080/00087114.2016.1169089.
- Bianco, P.G, Aprea, G., Balletto, E., Capriglione, T., Fulgione, D., & Odierna, G. (2004). The karyology of the cyprinid genera Scardinius and Rutilus in southern Europe. Ichthyological Research, 51, 274-278.
- Boron, A. (1999). Banded karyotype of spined loach Cobitis taenia and triploid Cobitis from Poland. Genetica, 105, 293-300.
- Boron, A., Porycka, K., Ito, D., Abe, S., & Kirtiklis, L. (2009). Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, Cyprinidae) using chromosome banding and FISH with rDNA. Genetica, 135, 199-207.
- Collares-Pereira, M.J. (1992). In vivo direct chromosome preparation (Air Drying Technique). 1st International Workshop on Fish Cytogenetic Techniques, Concarneau, pp. 15-20.
- Ergene, S., Karahan, A., & Kuru, M. (2010). Cytogenetic analysis of Pseudophoxinus antalyae, Bogustkaya, 1992 (Pisces: Cyprinidae) from the Eastern Mediterranean River Basin. Turkey. Turkish Journal of Zoology, 34(1), 111-117.
- Gaffaroğlu, M., Ayata, M.K., Ünal, S. & Yüksel, E. (2014). Chromosomal analysis of Pseudophoxinus elizavetae Bogutskaya, Kucuk and Atalay, 2007 (Teleostei, Cyprinidae) from Anatolia. FABA2014: International Symposium on Fisheries and Aquatic Science; 25-27 September; Trabzon.
- Gromicho, M. & Collares-Pereira, M.J. (2004). Polymorphism of major ribosomal gene chromosomal sites (NORphenotypes) in the hybridogenetic fish Squalius alburnoides complex (Cyprinidae) assessed through crossing experiments. Genetica, 122, 291-302.
- Güçlü, S.S., & Küçük, F. (2017). Endemic species of the genus Pseudophoxinus (Teleostei: Cyprinidae) in Anatolia and distribution areas. First International Symposium on Limnology and Freshwater Fisheries. 4-6 October, 2017, Eğirdir, Turkey.
- Kapuscinski, J. (1995). DAPI: a DNA-specific fluorescent probe. Biotechnic and Histochemistry, 70(5), 220-33.
- Karasu, M. (2009). Pseudophoxinus firati (Pisces:Cyprinidae)’nin karyotip özellikleri. Gazi Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Ankara.
- Karasu, M., Yüksel, E., & Gaffaroğlu, M. (2011). Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük, & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Turkey. Turkish Journal of Zoology, 35(6), 865-868. http://doi:10.3906/zoo-0912-129.
- Kirtiklis, L., Porycka, K., Boroñ, A., Coutanceau, J.P., & Dettai, A. (2010). Use of the chromosomal co-location of the minor 5S and the major 28S rDNA as a cytogenetic marker within the genus Leuciscus (Pisces, Cyprinidae). Folia biologica (Kraków), 58, 3-4. doi:10.3409/fb58_3-4.245-249.
- Mani, I., Kumar, R., Singh, M., Kushwaha, B., Nagpure, N.S., Srivastava, P.K., Murmu, K., Rao, D.S.K., & Lakra, W.S. (2009). Karyotypic diversity and evolution of seven mahseer species (Cyprinidae) from India. Journal of Fish Biology, 75, 1079-1091
- Mayr, B., Rab, P., & Kalat, M. (1986). NORs and counterstain-enhanced fluorescence studies in Cyprinidae of different ploidy level. Genetica, 69(2), 111-118.
- Monteiro, R., Carvalho, C., & Collares-Pereira, M.J. (2009). Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister species I. lusitanicum. Genetics and Molecular Biology, 32(2), 268-275.
- Nabais, C., Rampin, M., & Collares-Pereira, M.J. (2013). Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis and S. torgalensis (Teleostei, Cyprinidae), from the Iberian Peninsula. Comparative Cytogenetics, 7(1), 33-42.
- Rab, P., Rabova, M., Economidis, P.S., & Triantaphyllidis, C. (2000). Banded karyotype of the Greek endemic cyprinid fish. Pachychilon macedonicum. Ichthyological Reserarch, 47(1), 107-110.
- Rab, P., Karakousis, M.Y., Rabova, M., & Economidis, P.S. 1996. Banded karyotype of the cyprinid fish Leuciscus borysthenicus. Ichthyological Research, 43(4), 463-468.
- Rabova, M., Rab, P., Ozouf-Costaz, C., Ene, C., & Wanzebock, J. (2003). Comparative cytogenetics and chromosomal characteristics of ribosomal DNA in the fish genus Vimba (Cyprinidae). Genetica, 118, 83-91.
- Saç, G., Özuluğ, M., Elp, M., Gaffaroğlu, M., Ünal, S., Karasu Ayata, M., Kaya, C., & Freyhof, J. (2019). New records of Pseudophoxinus firati from Turkey (Teleostei: Leuciscidae). Journal of Applied Ichthyology, 1, 1-6. https://doi. org/10.1111/jai.13869.
- Sola, L., Rossi, A.R., Iaselli, V., Rasch, E.M., & Monaco, P.J. (1992). Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana mexicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenetics and Cell Genetics, 60, 229-235. doi: 10.1159/000133346.
- Unal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369.
- Ünal, S., Gaffaroğlu, M., Ayata, M.K., & Yüksel, E. (2014). Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae). Comparative Cytogenetics, 8(4), 249-257. http://doi: 10.3897/CompCytogen.v8i4.7623.
- Valic, D., Kapetanovıć, D., Zanella, D., Mrakovčıć, M., Teskeredžıć, E., Besendorfer, V., Rabova, M., & Rab, P. (2010). The karyotype and NOR phenotype of Telestes ukliva (Cyprinidae). Folia Zoologica, 59(2), 169-173.
There are 26 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Articles |
| Authors | |
| Project Number | SYO.A4.18.001 |
| Publication Date | June 1, 2020 |
| Published in Issue | Year 2020 Volume: 16 Issue: 2 |
