Year 2016, Volume 33, Issue 3, Pages 233 - 240 2016-08-15

Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum
Ünal İspir1 • Cebrahil Türk2 • Muammer Kırıcı3* • M.Reşit Taysı3 • Mikail Özcan4 1İnönü Üniversitesi, Su Ürünleri Fakültesi, Malatya, Türkiye 2 Bingöl Üniversitesi, Genç Meslek Yüksek Okulu, Su Ürünleri Programı, Bingöl, Türkiye 3 Bingöl Üniversitesi, Ziraat Fakültesi, Su Ürünleri Bölümü, Bingöl, Türkiye 4Kahramanmaraş Sütçü İmam Üniversitesi, Ziraat Fakültesi, Su Ürünleri Bölümü, Kahramanmaraş, Türkiye

Ünal İspir [1] , Cebrahil Türk [2] , Muammer Kırıcı [3] , M. Reşit Taysı [4] , Mikail Özcan [5]

540 1209

In this study, survival rate of rainbow trout (Oncorhynchus mykiss), fed for 28 days with sodium alginate microparticles containing Flavobacterium psychrophilum inactivated with formalin, against diseases is determined. The fishes are divided into six groups in terms of feeding method; control group, sodium alginated group, fishmeals including 2.5 and 10 mg/g F. psychrophilum, fishmeals of 2.5 and 10 mg/g F. psychrophilum encapsulated with alginate microparticles. Following by oral immunization, experimental infection is created by bathing them in 3.2x107 CFU/ml F. psychrophilum for 1 hour at 53. and 87. days and relative percent survival (RPS) rates are determined. At fifth third day of immunization, while mortality rate of 79.2% is observed for control group, the highest RPS value of 62.1 is found for the group fed with 10 mg/g F. psychrophilum alginate microparticles. At eighty seventh day in infectious F. psychrophilum, while control group’s mortality rate is found 74.8, the rest of groups RPS rates are determined 30.48, 35.3, 40.10, 50.25 and 56.68 respectively. Based on these findings, it can be asserted that protectiveness of fishes, fed with fishmeal encapsulated with sodium alginate microparticles by using inactivated bacterial cells, is not achieved at a sufficient level.

Keywords: Rainbow trout, Flavobacterium psychrophilum, sodium alginate microparticles, relative percent survival rates

Bu çalışmada, formalin ile inaktive edilmiş Flavobacterium psychrophilum'un sodyum aljinat ile kaplanmış mikropartiküller ile 28 gün için beslenen gökkuşağı alabalık (Oncorhynchus mykiss)'larının hastalığa karşı koruma oranları tespit edildi. Balıklar kontrol, sodyum aljinatlı grup, 2.5 ve 10 mg/g F. psychrophilum içeren yem, 2.5 ve 10 mg/g F. psychrophilum’un aljinat mikropartikülleri ile kapsüllendiği yem ile beslenen olmak üzere altı gruba ayrıldı. Oral immunizasyonu takiben 53 ve 87. gün 3.2x107 CFU/ml F. psychrophilum ile 1 saat banyo yaptırılarak deneysel enfeksiyon oluşturuldu ve nispi hayatta kalma (Relative percent survival, RPS) oranları belirlendi. İmmunizasyonun 53. gününde kontrol grubu balıklarında %79.2 oranında mortalite gözlenirken en yüksek RPS değeri 62.1 ile 10 mg/g F. psychrophilum’un aljinat mikropartikülleri ile beslenen grupta tespit edildi. F. psychrophilum ile 87. günde yapılan enfeksiyonda kontrol grubunda 74.8 mortalite oranı bulunmuşken diğer gruplardaki RPS oranı sırasıyla 30.48, 35.30, 40.10, 50.25 ve 56.68 olarak belirlendi. Bu bulgulara göre inaktive bakteri hücreleri kullanılarak sodyum aljinat mikropartikülleri ile kapsüllenen yem ile beslenen balıklarda koruyuculuğun yeterli düzeyde oluşmadığı ileri sürülebilir
Gökkuşağı alabalığı, Flavobacterium psychrophilum, sodyum aljinat mikropartikülleri, nispi hayatta kalma oranları
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Journal Section Articles
Authors

Author: Ünal İspir

Author: Cebrahil Türk

Author: Muammer Kırıcı

Author: M. Reşit Taysı

Author: Mikail Özcan

Dates

Publication Date: August 15, 2016

Bibtex @ { egejfas258600, journal = {Ege Journal of Fisheries and Aquatic Sciences}, issn = {1300-1590}, eissn = {2148-3140}, address = {Ege University}, year = {2016}, pages = {233 - 240}, doi = {10.12714/egejfas.2016.33.3.07}, title = {Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum}, key = {cite}, author = {İspir, Ünal and Türk, Cebrahil and Kırıcı, Muammer and Taysı, M. Reşit and Özcan, Mikail} }
APA İspir, Ü , Türk, C , Kırıcı, M , Taysı, M , Özcan, M . (2016). Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum. Ege Journal of Fisheries and Aquatic Sciences, 33 (3), 233-240. DOI: 10.12714/egejfas.2016.33.3.07
MLA İspir, Ü , Türk, C , Kırıcı, M , Taysı, M , Özcan, M . "Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum". Ege Journal of Fisheries and Aquatic Sciences 33 (2016): 233-240 <http://www.egejfas.org/egejfas/article/258600>
Chicago İspir, Ü , Türk, C , Kırıcı, M , Taysı, M , Özcan, M . "Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum". Ege Journal of Fisheries and Aquatic Sciences 33 (2016): 233-240
RIS TY - JOUR T1 - Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum AU - Ünal İspir , Cebrahil Türk , Muammer Kırıcı , M. Reşit Taysı , Mikail Özcan Y1 - 2016 PY - 2016 N1 - doi: 10.12714/egejfas.2016.33.3.07 DO - 10.12714/egejfas.2016.33.3.07 T2 - Ege Journal of Fisheries and Aquatic Sciences JF - Journal JO - JOR SP - 233 EP - 240 VL - 33 IS - 3 SN - 1300-1590-2148-3140 M3 - doi: 10.12714/egejfas.2016.33.3.07 UR - https://doi.org/10.12714/egejfas.2016.33.3.07 Y2 - 2019 ER -
EndNote %0 Ege Journal of Fisheries and Aquatic Sciences Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum %A Ünal İspir , Cebrahil Türk , Muammer Kırıcı , M. Reşit Taysı , Mikail Özcan %T Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum %D 2016 %J Ege Journal of Fisheries and Aquatic Sciences %P 1300-1590-2148-3140 %V 33 %N 3 %R doi: 10.12714/egejfas.2016.33.3.07 %U 10.12714/egejfas.2016.33.3.07
ISNAD İspir, Ünal , Türk, Cebrahil , Kırıcı, Muammer , Taysı, M. Reşit , Özcan, Mikail . "Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum". Ege Journal of Fisheries and Aquatic Sciences 33 / 3 (August 2016): 233-240. https://doi.org/10.12714/egejfas.2016.33.3.07
AMA İspir Ü , Türk C , Kırıcı M , Taysı M , Özcan M . Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum. EgeJFAS. 2016; 33(3): 233-240.
Vancouver İspir Ü , Türk C , Kırıcı M , Taysı M , Özcan M . Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum. Ege Journal of Fisheries and Aquatic Sciences. 2016; 33(3): 240-233.